| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Thomas P (16)
|
OHRCDB |
54.25 |
54.75 |
0.50 |
| 2 |
Wetta, Lance A (17)
|
OHGMVY |
55.74 |
57.15 |
1.41 |
| 3 |
Carmack, Aaron B (16)
|
OHMCY |
57.32 |
58.94 |
1.62 |
| 4 |
Hagan, Josh K (15)
|
OHCFY |
57.66 |
59.05 |
1.39 |
| 5 |
Beach, Colin J (17)
|
OHCFY |
57.22 |
59.45 |
2.23 |
| 6 |
Knopf, Luke S (16)
|
OHCFY |
55.77 |
59.64 |
3.87 |
| 7 |
Stuber, Matthew J (16)
|
OHCFY |
58.35 |
1:00.44 |
2.09 |
| 8 |
Hooper, Thomas J (16)
|
OHLAKY |
58.84 |
1:01.86 |
3.02 |
| 9 |
Villa, Sebastian (16)
|
OHKEY |
1:02.09 |
1:02.83 |
0.74 |
| 10 |
Chivington, Wesley D (15)
|
OHCFY |
1:02.01 |
1:03.68 |
1.67 |
| 11 |
Crank, Colin (16)
|
OHGMVY |
1:05.39 |
1:04.25 |
-1.14 |
| 12 |
Morris, Aubrey R (18)
|
OHSPY |
1:01.32 |
1:04.39 |
3.07 |
| 13 |
Collins, Seamus C (15)
|
OHRCDB |
1:10.54 |
1:07.17 |
-3.37 |
| 14 |
Rocheleau, Noah B (17)
|
OHLAKY |
1:00.67 |
1:07.27 |
6.60 |
| 15 |
Stone, Gabriel D (15)
|
OHKEY |
1:05.71 |
1:07.58 |
1.87 |
| 16 |
Mihaljevic, Joshua J (15)
|
OHRCDB |
1:05.09 |
1:07.66 |
2.57 |
| 17 |
Mayhan, Carter J (15)
|
OHSPY |
1:03.87 |
1:08.86 |
4.99 |
| 18 |
Schierenbeck, Camden L (16)
|
OHRCDB |
|
1:09.11 |
- |