| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hess, Brooke A (8)
|
OZHHST |
39.63 |
37.50 |
-2.13 |
| 2 |
Foster, Kendall R (8)
|
ILSPY |
39.08 |
39.02 |
-0.06 |
| 3 |
Harris, Audrey G (7)
|
OZHHST |
44.14 |
41.78 |
-2.36 |
| 4 |
Ozyurt, Mary K (8)
|
ILSPY |
44.91 |
42.90 |
-2.01 |
| 5 |
Meier, Kenna R (8)
|
ILSPY |
|
43.99 |
- |
| 6 |
St. Juliana, Audra L (7)
|
OZHHST |
1:00.78 |
53.18 |
-7.60 |
| 7 |
Ehmen, Josie K (8)
|
ILSPY |
56.88 |
54.90 |
-1.98 |
| 8 |
Hilligoss, Madalyn A (8)
|
ILSPY |
1:00.70 |
58.81 |
-1.89 |
| 9 |
Hacke, Phoebe J (7)
|
ILSPY |
57.51 |
58.84 |
1.33 |
| 10 |
Stivers, Dahlia W (7)
|
ILSPY |
1:05.37 |
1:12.59 |
7.22 |
|
Hockenyos, Elizabeth G (8)
|
ILSPY |
|
NS |
- |