| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trinh, Lili A (17)
|
PVNCAP |
5:19.18 |
5:23.19 |
4.01 |
| 2 |
Nguyen, Lien J (15)
|
PVPM |
5:20.63 |
5:23.26 |
2.63 |
| 3 |
Smith, Lauren C (17)
|
PVNCAP |
5:33.45 |
5:28.83 |
-4.62 |
| 4 |
Harris, Savannah B (15)
|
PVNCAP |
5:39.55 |
5:29.01 |
-10.54 |
| 5 |
Wittholz, Katherine E (17)
|
MDCAA |
5:42.10 |
5:40.66 |
-1.44 |
| 6 |
Morris, Payton E (16)
|
PVNCAP |
5:49.53 |
5:44.32 |
-5.21 |
| 7 |
Greco, Mia M (16)
|
MDCAA |
|
5:53.80 |
- |
| 8 |
Pecora, Claire E (15)
|
MDCAA |
6:03.95 |
6:01.88 |
-2.07 |
| 9 |
Johnson, Avery L (15)
|
PVNCAP |
6:04.76 |
6:02.17 |
-2.59 |
| 10 |
McGillivray-Stubbs, Maren El (16)
|
MDCAA |
5:59.50 |
6:06.35 |
6.85 |
| 11 |
Johnson, Lilah G (16)
|
PVPM |
|
6:33.25 |
- |
| 12 |
Heitman, Morgan L (17)
|
PVPM |
|
6:46.21 |
- |
|
Johnson, Jordan N (17)
|
PVNCAP |
5:59.99 |
DQ |
- |