| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Detrisac, Ainsley M (8)
|
PVMACH |
53.07 |
53.05 |
-0.02 |
| 2 |
Trencevska, Leila (8)
|
PVRY |
51.83 |
53.24 |
1.41 |
| 3 |
Buonforte, Lilia R (8)
|
PVMACH |
|
54.31 |
- |
| 4 |
Djoubrailov, Sarah G (8)
|
PVMACH |
|
54.55 |
- |
| 5 |
Roth, Ava G (8)
|
PVMACH |
56.88 |
56.59 |
-0.29 |
| 6 |
Bock, Katharina S (8)
|
PVSDS |
1:00.95 |
57.37 |
-3.58 |
| 7 |
Schneidau, Rayne E (8)
|
PVSDS |
59.33 |
1:00.68 |
1.35 |
| 8 |
Perlin, Miriam S (8)
|
PVSDS |
1:05.08 |
1:03.06 |
-2.02 |
| 9 |
Adams, Ashleigh J (8)
|
PVMACH |
|
1:03.60 |
- |
| 10 |
Cowie, Reese G (7)
|
PVSDS |
1:04.72 |
1:04.99 |
0.27 |
| 11 |
Ghosh, Ava (7)
|
PVSDS |
|
1:06.80 |
- |
| 12 |
Wilkins, Sophia L (8)
|
PVSDS |
1:09.13 |
1:12.65 |
3.52 |
| 13 |
Lee, Heidi C (7)
|
PVSDS |
1:23.55 |
1:25.29 |
1.74 |
|
Nadramija Cardenas, Olivia (8)
|
PVSDS |
|
DQ |
- |
|
Tanapornsakul, Rae (7)
|
PVSDS |
|
DQ |
- |