| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Almassian, Laleh R (10)
|
PVNCAP |
46.28 |
39.04 |
-7.24 |
| 2 |
Johnson, Lauren E (10)
|
PVBWST |
45.41 |
39.58 |
-5.83 |
| 3 |
Lu, Sophia A (10)
|
PVPM |
|
40.17 |
- |
| 4 |
Zimmermann, Merick A (10)
|
MDCAA |
40.97 |
40.24 |
-0.73 |
| 5 |
Radford, Callie A (10)
|
PVNCAP |
42.98 |
40.99 |
-1.99 |
| 6 |
Young, Victoria N (10)
|
PVSTJS |
54.86 |
41.33 |
-13.53 |
| 7 |
Casagrande, Claire S (10)
|
MDCAA |
52.96 |
41.42 |
-11.54 |
| 8 |
Moretz, Courtney K (10)
|
PVSTJS |
|
42.92 |
- |
| 9 |
Ketler, Hannah J (10)
|
MDCAA |
41.97 |
44.83 |
2.86 |
| 10 |
Musni, Trisha M (10)
|
PVDRAG |
51.20 |
46.47 |
-4.73 |
| 11 |
Patel, Priya R (10)
|
PVDRAG |
49.71 |
47.48 |
-2.23 |
| 12 |
Qin, Zhiyue Z (9)
|
PVNCAP |
|
47.64 |
- |
| 13 |
Sumrow, Eliza R (9)
|
PVNCAP |
|
48.40 |
- |
| 14 |
Hernandez, Rylee J (10)
|
PVBWST |
56.08 |
51.76 |
-4.32 |
| 15 |
Washburn, Rosalia G (9)
|
PVNCAP |
|
52.09 |
- |
| 16 |
Roembke, Reagan S (10)
|
PVNCAP |
|
52.71 |
- |
| 17 |
Tanner, Tirzah E (10)
|
MDCAA |
55.90 |
54.33 |
-1.57 |
| 18 |
Conway, Georgia W (9)
|
PVNCAP |
|
56.01 |
- |
| 19 |
Ye, Sophie X (10)
|
PVNCAP |
|
1:01.93 |
- |
|
Thomas, Sylvie E (9)
|
PVNCAP |
|
DQ |
- |
|
McKoy, Daniella M (9)
|
PVNCAP |
|
NS |
- |
|
McKoy, Gabriella M (10)
|
PVNCAP |
1:00.00 |
NS |
- |
|
North, Kylie J (9)
|
PVNCAP |
|
NS |
- |
|
Monterrey, Gabriella E (10)
|
PVFAST |
|
NS |
- |