| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Badger, Lauren M (17)
|
PVTIBU |
53.03 |
54.11 |
1.08 |
| 2 |
Martin, Allison M (18)
|
PVFISH |
51.29 |
54.40 |
3.11 |
| 3 |
Cleal, Lilly M (17)
|
PVFISH |
54.26 |
54.51 |
0.25 |
| 4 |
Moschella, Clara (15)
|
PVYORK |
57.51 |
54.89 |
-2.62 |
| 5 |
Scherber, Aleigha C (17)
|
PVMAKO |
54.92 |
54.94 |
0.02 |
| 6 |
Modder, Kathleen F (16)
|
PVFISH |
55.55 |
55.77 |
0.22 |
| 7 |
Owen, Veronika N (14)
|
PVUN |
55.29 |
55.78 |
0.49 |
| 8 |
Wargo, Laura M (16)
|
PVUN |
54.27 |
55.99 |
1.72 |
| 9 |
Wilson, Emerson L (15)
|
PVMAKO |
58.67 |
56.36 |
-2.31 |
| 10 |
Gregory, Ashley B (16)
|
PVFISH |
59.63 |
56.75 |
-2.88 |
| 11 |
Siraj, Hannah (13)
|
PVUN |
56.67 |
56.92 |
0.25 |
| 12 |
Judge, Catherine S (16)
|
PVTIBU |
57.09 |
57.19 |
0.10 |
| 13 |
Brooks, Parker D (15)
|
PVMAKO |
59.28 |
57.22 |
-2.06 |
| 14 |
Deer, Abigail M (18)
|
PVFISH |
1:01.60 |
57.31 |
-4.29 |
| 15 |
May, Mikayla K (16)
|
PVMAKO |
57.55 |
57.37 |
-0.18 |
| 16 |
Do, Clara T (15)
|
PVFISH |
57.29 |
57.45 |
0.16 |
| 17 |
Bailey, Caroline S (15)
|
PVYORK |
58.30 |
57.57 |
-0.73 |
| 18 |
Lewis, Sydney C (15)
|
PVFISH |
1:00.90 |
57.80 |
-3.10 |
| 19 |
Baker, Korelle Z (17)
|
PVTIBU |
57.23 |
59.19 |
1.96 |
| 20 |
Ford, Jacqueline M (18)
|
PVFISH |
1:06.14 |
1:00.25 |
-5.89 |
| 21 |
Dean, Selah G (17)
|
PVFISH |
58.12 |
1:01.18 |
3.06 |
| 22 |
Hoover, Sophia R (15)
|
PVTIBU |
1:01.33 |
1:01.24 |
-0.09 |
| 23 |
Williams, Madeleine P (18)
|
PVUN |
1:01.53 |
1:01.81 |
0.28 |
| 24 |
Salomons, Catherine A (15)
|
PVSTJS |
1:04.01 |
1:02.51 |
-1.50 |
| 25 |
Brandt, Sophia A (17)
|
PVTIBU |
1:01.11 |
1:03.56 |
2.45 |
| 26 |
McGowan, Genevieve E (15)
|
PVUN |
1:04.27 |
1:04.69 |
0.42 |
| 27 |
Saunders, Alicia B (15)
|
PVUN |
1:06.75 |
1:04.86 |
-1.89 |
| 28 |
Williams, Meaghan N (14)
|
PVUN |
1:11.19 |
1:09.94 |
-1.25 |
|
Xie, Manda (16)
|
PVFISH |
1:07.16 |
NS |
- |