| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Frerichs, Greta L (11)
|
NIHHSC |
48.34 |
47.25 |
-1.09 |
| 2 |
Keefe, Stephen N (12)
|
NIHHSC |
48.51 |
47.69 |
-0.82 |
| 3 |
Cerasani, Gabrielle F (10)
|
NISWAT |
49.76 |
53.12 |
3.36 |
| 4 |
Gilcrist, Gracie M (11)
|
NISWAT |
1:13.09 |
58.87 |
-14.22 |
| 5 |
Cully, Ella M (11)
|
NIHHSC |
|
1:06.85 |
- |
| 6 |
Dunn, Mary T (11)
|
NIHHSC |
|
1:08.20 |
- |
| 7 |
Lookenhouse, Annabelle K (12)
|
NISWAT |
|
1:19.69 |
- |
|
Abram-Hopkins, Wesley A (10)
|
NIAAAC |
|
DQ |
- |
|
Clinch, Jonathan T (10)
|
NIAAAC |
|
DQ |
- |
|
Stenger, Cooper J (8)
|
NIHHSC |
|
DQ |
- |
|
Cerasani, Asa J (9)
|
NISWAT |
|
DQ |
- |
|
Safford, Christian T (11)
|
NISWAT |
1:11.93 |
DQ |
- |
|
Hartung, Ava M (9)
|
NIHHSC |
|
NS |
- |
|
Maybee, Marlee M (10)
|
NISAAC |
58.65 |
NS |
- |