| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Uhteg, Julia D (12)
|
NISWAT |
32.62 |
31.66 |
-0.96 |
| 2 |
Cerasani, Gabrielle F (11)
|
NISWAT |
33.53 |
32.85 |
-0.68 |
| 3 |
Dickens, Jennifer E (12)
|
NIGVST |
33.83 |
33.64 |
-0.19 |
| 4 |
Zywiczynski, Gracie A (12)
|
NIAAAC |
37.66 |
33.69 |
-3.97 |
| 5 |
Ballantyne, Riley E (12)
|
NISWAT |
35.27 |
35.15 |
-0.12 |
| 6 |
Gavin, Lily L (11)
|
NISWAT |
35.77 |
35.77 |
0.00 |
| 7 |
Uhteg, Ella M (11)
|
NISWAT |
37.32 |
36.14 |
-1.18 |
| 8 |
Budinger, Emily A (11)
|
NIGVST |
38.11 |
37.04 |
-1.07 |
| 9 |
Kinnicutt, Grace A (11)
|
NIGVST |
37.00 |
37.63 |
0.63 |
| 10 |
Melnik, Kirsten (11)
|
NISWAT |
37.78 |
38.28 |
0.50 |
| 11 |
Durnford, Riley A (11)
|
NIGWST |
42.33 |
38.72 |
-3.61 |
| 12 |
DeBoy, Kortney B (12)
|
NISAAC |
39.94 |
38.90 |
-1.04 |
| 13 |
Capozzi, Alivia M (11)
|
NIGWST |
41.02 |
38.91 |
-2.11 |
| 14 |
Hediger, Lileigh G (11)
|
NIAAAC |
40.47 |
39.60 |
-0.87 |
| 15 |
Westbrook, Danika G (11)
|
NISWAT |
42.41 |
40.43 |
-1.98 |
| 16 |
Rounds, Jade E (11)
|
NIGVST |
38.32 |
41.15 |
2.83 |
| 17 |
VanEtten, Megan T (11)
|
NIGVST |
40.81 |
41.76 |
0.95 |
| 18 |
Ireland, Alexis M (11)
|
NIGVST |
47.25 |
46.00 |
-1.25 |
| 19 |
Boussa, Serena M (11)
|
NIGVST |
44.95 |
46.69 |
1.74 |
| 20 |
John, Lauren E (11)
|
NISAAC |
52.99 |
49.28 |
-3.71 |
| 21 |
Culbertson, Morgan E (11)
|
NIGVST |
59.55 |
53.37 |
-6.18 |