| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Uhteg, Julia D (12)
|
NISWAT |
33.23 |
32.62 |
-0.61 |
| 2 |
Herman, Tyyetta R (11)
|
NIFKVL |
33.42 |
33.49 |
0.07 |
| 3 |
Cerasani, Gabrielle F (11)
|
NISWAT |
33.53 |
33.71 |
0.18 |
| 4 |
Ballantyne, Riley E (12)
|
NISWAT |
35.78 |
35.27 |
-0.51 |
| 5 |
Rosenswie, Emma G (12)
|
NISWAT |
36.78 |
36.28 |
-0.50 |
| 6 |
Melnik, Kirsten (11)
|
NISWAT |
38.23 |
38.78 |
0.55 |
| 7 |
DeBoy, Kortney B (11)
|
NISAAC |
41.73 |
39.94 |
-1.79 |
| 8 |
Capozzi, Alivia M (11)
|
NIGWST |
42.91 |
42.03 |
-0.88 |
| 9 |
Hediger, Lileigh G (11)
|
NIAAAC |
40.47 |
44.39 |
3.92 |
| 10 |
Maybee, Marlee M (11)
|
NISAAC |
45.45 |
45.41 |
-0.04 |
| 11 |
Rohwer, Kelsey L (11)
|
NISAAC |
42.07 |
45.79 |
3.72 |
| 12 |
Weiss, Emmie S (12)
|
NISWAT |
48.55 |
51.74 |
3.19 |
|
McAuley, Catherine G (11)
|
NIFKVL |
41.75 |
NS |
- |
|
Mentley-Peters, Willow S (11)
|
NIGWST |
43.69 |
NS |
- |
|
Bennett, Leah E (12)
|
NIHHSC |
|
NS |
- |
|
Spacciapolli, Julianna M (11)
|
NIHHSC |
35.07 |
NS |
- |