| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Regenwetter, Jake (14)
|
ILHEAT |
59.49 |
55.43 |
-4.06 |
| 2 |
Herrick, Charlie S (14)
|
ILMYST |
55.26 |
56.52 |
1.26 |
| 3 |
Boelens, Michael V (14)
|
ILSAGE |
58.59 |
57.56 |
-1.03 |
| 4 |
Deats, Nolan (14)
|
ILSTRY |
58.89 |
58.73 |
-0.16 |
| 5 |
Taylor, Camden J (13)
|
ILBVDY |
1:00.74 |
59.49 |
-1.25 |
| 6 |
Schlueter, Dom C (14)
|
ILSAGE |
58.24 |
1:00.01 |
1.77 |
| 7 |
Taller, Zackary M (14)
|
ILSAGE |
58.32 |
1:00.13 |
1.81 |
| 8 |
Phillips, Owen (14)
|
ILOTTY |
1:03.19 |
1:01.04 |
-2.15 |
| 9 |
Alhamwi, Karim (13)
|
ILMYST |
1:04.86 |
1:01.11 |
-3.75 |
| 10 |
Stevenson, Riley (13)
|
KNCY |
1:01.40 |
1:01.39 |
-0.01 |
| 11 |
Miller, Nolan P (14)
|
ILHEAT |
1:03.41 |
1:01.43 |
-1.98 |
| 12 |
Johnson, Noah W (13)
|
ILDCST |
1:01.48 |
1:02.54 |
1.06 |
| 12 |
Lowery, Christopher A (13)
|
ILILVY |
1:01.03 |
1:02.54 |
1.51 |
| 14 |
VanderSchee, Jared I (14)
|
ILDCST |
1:02.33 |
1:02.72 |
0.39 |
| 15 |
Watson, William A (13)
|
ILSAGE |
1:03.69 |
1:02.89 |
-0.80 |
| 15 |
Burgin, Mitchell W (13)
|
ILSPY |
1:03.10 |
1:02.89 |
-0.21 |
| 17 |
Perry, Duncan M (14)
|
ILOTTY |
1:02.78 |
1:03.19 |
0.41 |
| 18 |
Vye, Christopher j (13)
|
ILMYST |
1:05.79 |
1:04.48 |
-1.31 |
| 19 |
Gramer, Jacob D (13)
|
ILDCST |
1:04.37 |
1:05.05 |
0.68 |
| 20 |
Ehmen, Rowan C (13)
|
ILSPY |
1:03.45 |
1:05.07 |
1.62 |
| 21 |
Mestan, Ben M (14)
|
ILJETS |
1:06.42 |
1:05.18 |
-1.24 |
| 22 |
Schwieters, Luke R (14)
|
ILBRRY |
1:05.96 |
1:05.70 |
-0.26 |
| 23 |
Eberle, Eli (13)
|
ZZCANY |
1:05.89 |
1:05.81 |
-0.08 |
| 24 |
Allen, Grant S (13)
|
ILSPY |
1:05.17 |
1:06.03 |
0.86 |
| 25 |
Williams, Aidan T (13)
|
ILHEAT |
1:05.17 |
1:06.20 |
1.03 |
| 26 |
Dempsey, Maddox C (14)
|
ILHEAT |
1:05.40 |
1:06.91 |
1.51 |
| 27 |
Myers, Leo X (14)
|
ILPALA |
1:05.06 |
1:07.10 |
2.04 |
| 28 |
Foster, Luke (14)
|
KNCY |
1:06.37 |
1:07.29 |
0.92 |
| 29 |
Lucas, Jacob (14)
|
ILSTRY |
1:06.35 |
1:07.66 |
1.31 |
| 30 |
Su, Herschel (13)
|
ILJETS |
1:05.42 |
1:08.95 |
3.53 |