| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vervlied, Zachary T (14)
|
INLCB |
26.52 |
26.40 |
-0.12 |
| 2 |
Dhana, Jack B (14)
|
INLCB |
29.51 |
27.43 |
-2.08 |
| 3 |
Gabel, Shane T (14)
|
ILLWSA |
29.85 |
28.17 |
-1.68 |
| 4 |
Miles, Matthew T (14)
|
INLCB |
38.15 |
28.90 |
-9.25 |
| 5 |
Venslauskas, Kasparas (14)
|
ILRA |
31.17 |
28.93 |
-2.24 |
| 6 |
Jacob, Alejandro J (14)
|
ILLIFE |
33.17 |
29.21 |
-3.96 |
| 7 |
Martillo, Elliot (13)
|
ILUNAT |
32.51 |
29.59 |
-2.92 |
| 8 |
Weber, Hayden R (14)
|
INLCB |
31.21 |
29.96 |
-1.25 |
| 9 |
Gallagher, Colin S (14)
|
INLCB |
33.94 |
30.61 |
-3.33 |
| 10 |
McCormack, Jacob M (14)
|
ILLWSA |
33.25 |
31.02 |
-2.23 |
| 11 |
Mroz, John J (13)
|
ILLWSA |
|
31.18 |
- |
| 12 |
Petricca, Daniel D (14)
|
ILRA |
|
31.63 |
- |
| 13 |
Nyobuya, Paschal E (13)
|
INMSC |
|
31.99 |
- |
| 14 |
Bulfer, Jack T (14)
|
INLCB |
37.83 |
32.04 |
-5.79 |
| 15 |
Ace, Nicholas C (14)
|
INLCB |
41.90 |
32.11 |
-9.79 |
| 16 |
Lambert, Tyler J (13)
|
ILLWSA |
|
33.84 |
- |
| 17 |
Bach, Tyler C (14)
|
ILNAC |
40.62 |
35.08 |
-5.54 |
| 18 |
Anderson, Gabriel K (13)
|
INLCB |
|
36.08 |
- |
| 19 |
Martinez, Sebastian A (13)
|
INMSC |
39.02 |
36.75 |
-2.27 |
|
Kurek, Dominik S (13)
|
ILRA |
33.91 |
NS |
- |