| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Little, Isabella R (11)
|
VANOVA |
30.58 |
28.99 |
-1.59 |
| 2 |
Ancona, Siena L (11)
|
PVNCAP |
31.43 |
30.46 |
-0.97 |
| 3 |
Menz, Madeline M (11)
|
PVNCAP |
33.08 |
31.49 |
-1.59 |
| 4 |
Hotem, Kaitlyn R (11)
|
VANOVA |
32.37 |
31.73 |
-0.64 |
| 5 |
Trinh, Lili A (11)
|
PVNCAP |
33.44 |
31.98 |
-1.46 |
| 6 |
Webb, Sarah G (11)
|
VANOVA |
33.12 |
32.18 |
-0.94 |
| 7 |
Strabuk, Savanah G (11)
|
MRLIAC |
32.55 |
32.20 |
-0.35 |
| 8 |
Cypher, Ella M (11)
|
PVNCAP |
32.94 |
32.53 |
-0.41 |
| 9 |
Hawkins, Mary-Stuart M (11)
|
VANOVA |
33.81 |
32.80 |
-1.01 |
| 10 |
Chen, Calista (11)
|
MRUN-L |
33.63 |
33.97 |
0.34 |
| 11 |
Schnappauf, Samantha G (11)
|
MRLIAC |
37.02 |
35.75 |
-1.27 |
| 12 |
McDonald, Mary J (11)
|
MRLIAC |
36.81 |
36.00 |
-0.81 |