| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harris, Savannah J (11)
|
VANOVA |
31.00 |
29.73 |
-1.27 |
| 2 |
Cushman, McKinley L (11)
|
PVNCAP |
30.62 |
30.52 |
-0.10 |
| 3 |
Munchel, Dylan R (11)
|
PVNCAP |
30.72 |
30.73 |
0.01 |
| 4 |
Liu, Shanshan (11)
|
VANOVA |
31.21 |
31.09 |
-0.12 |
| 5 |
Tang, Natalie J (11)
|
VANOVA |
31.71 |
31.14 |
-0.57 |
| 6 |
Dmochowski, Vivian Louise S (11)
|
PVNCAP |
30.58 |
31.38 |
0.80 |
| 7 |
Diaz, Una M (11)
|
MRLIAC |
33.08 |
31.63 |
-1.45 |
| 8 |
Edwards, Amelia L (11)
|
PVNCAP |
31.47 |
31.64 |
0.17 |
| 9 |
Tom, Kaitlyn L (11)
|
MRLIAC |
32.99 |
32.89 |
-0.10 |
| 10 |
Simons, Ashley R (11)
|
MRLIAC |
34.08 |
33.60 |
-0.48 |
| 11 |
Williams, Caroline H (11)
|
VANOVA |
35.74 |
34.48 |
-1.26 |
| 12 |
Stertsios, Martha M (11)
|
MRLIAC |
36.10 |
35.94 |
-0.16 |