| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reed, Ella E (14)
|
VACYAC |
2:36.97 |
2:38.79 |
1.82 |
| 2 |
Campbell, Tessa N (13)
|
VARAYS |
2:28.37 |
2:39.95 |
11.58 |
| 3 |
Ross, Marissa J (14)
|
VASTAT |
2:35.13 |
2:41.16 |
6.03 |
| 4 |
De Jong, Emma E (14)
|
VAVSTP |
2:31.49 |
2:43.25 |
11.76 |
| 5 |
Espinosa, Shalom K (13)
|
PVNCAP |
2:42.95 |
2:44.39 |
1.44 |
| 6 |
Patz, Sophia N (14)
|
MDSPRC |
2:41.16 |
2:44.83 |
3.67 |
| 7 |
Waite, Emily E (13)
|
VASTAT |
2:48.68 |
2:45.35 |
-3.33 |
| 8 |
Juhlin, Lindsay E (13)
|
VAPWSC |
2:40.92 |
2:45.99 |
5.07 |
| 9 |
Yu, Sophia (13)
|
VACYAC |
2:50.91 |
2:47.28 |
-3.63 |
| 10 |
Born, Sarah E (14)
|
VARAYS |
3:10.00 |
2:49.09 |
-20.91 |
| 11 |
Loder, Alexis G (14)
|
VASTAT |
2:50.05 |
2:49.32 |
-0.73 |
| 12 |
Perdomo, Cristal L (14)
|
VARAYS |
2:52.14 |
2:49.85 |
-2.29 |
| 13 |
Parker, Peyton R (14)
|
VARAYS |
2:53.68 |
2:51.93 |
-1.75 |
| 14 |
Pearson, Bridgette L (14)
|
VARAYS |
2:50.55 |
2:54.22 |
3.67 |
| 15 |
Niesent, Lauren G (14)
|
VARAYS |
2:56.18 |
2:55.73 |
-0.45 |
| 16 |
Luce, Annika G (13)
|
VARAYS |
2:51.77 |
2:58.25 |
6.48 |
| 17 |
Brown, Madison S (14)
|
VARAYS |
3:01.34 |
2:59.04 |
-2.30 |
| 18 |
Graham, Jean M (13)
|
VARAPP |
|
2:59.73 |
- |
| 19 |
Farabaugh, Kristen N (14)
|
VACYAC |
2:56.67 |
3:01.00 |
4.33 |
| 20 |
Brady, Caroline (14)
|
VATORP |
3:02.41 |
3:08.84 |
6.43 |
| 21 |
Ningcharoen, Nunthicha K (14)
|
VAPWSC |
|
3:10.76 |
- |
| 22 |
Parker, Mackenzie A (13)
|
MDSPRC |
3:12.19 |
3:15.26 |
3.07 |
| 23 |
Tusing, Natalee M (13)
|
VAVSTP |
3:28.89 |
3:21.46 |
-7.43 |
| 24 |
Fino, Grace E (13)
|
VATSU |
3:27.11 |
3:24.09 |
-3.02 |
| 25 |
Garcia, Madison O (14)
|
VATORP |
|
3:34.86 |
- |
|
Bain, Mattie J (13)
|
VATSU |
|
DQ |
- |
|
Nelson, Jordana M (14)
|
VASTAT |
2:59.97 |
NS |
- |
|
Meister, Emily L (14)
|
MDSPRC |
|
NS |
- |
|
Banks, Diana (13)
|
MDSPRC |
3:42.03 |
NS |
- |