| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Negron, Robert (17)
|
MABYST |
54.11 |
51.44 |
-2.67 |
| 2 |
Ventura, Stephen P (17)
|
MAYY |
54.24 |
53.34 |
-0.90 |
| 3 |
Laing, Sean P (16)
|
MAPAY |
53.86 |
53.69 |
-0.17 |
| 4 |
Zilch, Herman IV G (14)
|
AMGJY |
53.40 |
54.65 |
1.25 |
| 5 |
Scoboria, Christopher M (17)
|
MARY |
56.20 |
54.77 |
-1.43 |
| 6 |
Cunningham, Joshua L (14)
|
MARY |
55.54 |
55.47 |
-0.07 |
| 7 |
Griffith, Isaac (14)
|
AMINDY |
54.75 |
55.79 |
1.04 |
| 8 |
Malmstrom, Abigail M (14)
|
MAUMLY |
59.29 |
59.86 |
0.57 |
| 9 |
Niness, Mikayla J (15)
|
MAPAY |
59.64 |
59.96 |
0.32 |
| 10 |
Taylor, Samantha (15)
|
MABYST |
1:00.00 |
1:01.39 |
1.39 |
| 11 |
Burns, Delaney M (13)
|
MAUMLY |
59.93 |
1:01.90 |
1.97 |
| 12 |
Krotzer, Sabrina (18)
|
MABYST |
1:01.01 |
1:03.46 |
2.45 |
| 13 |
Brown, Rebecca M (17)
|
MARY |
1:02.79 |
1:04.70 |
1.91 |