| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Orlando, Peyton B (17)
|
MDFSC |
23.38 |
23.60 |
0.22 |
| 2 |
Johnson, Abbygail (13)
|
PVRY |
26.15 |
26.23 |
0.08 |
| 3 |
Snow, Selena G (16)
|
MDFSC |
25.28 |
27.00 |
1.72 |
| 4 |
Cherry, Ava E (15)
|
MDFSC |
26.41 |
27.20 |
0.79 |
| 5 |
Semanchik, Katherine A (16)
|
PVRY |
26.56 |
27.79 |
1.23 |
| 6 |
Babik, Finley E (14)
|
MDFSC |
26.52 |
28.04 |
1.52 |
| 7 |
Hanlon, Kelsey R (13)
|
MDFSC |
27.84 |
28.71 |
0.87 |
| 8 |
Walke, Caroline I (14)
|
PVRY |
29.40 |
28.81 |
-0.59 |
| 9 |
Abboud, Chloe M (13)
|
PVRY |
29.51 |
29.54 |
0.03 |
| 10 |
Bauer, Elizabeth H (18)
|
MDFSC |
29.27 |
29.69 |
0.42 |
| 11 |
Regan, Haley E (13)
|
MDFSC |
29.89 |
29.94 |
0.05 |
| 12 |
Montgomery, Paige R (16)
|
MDFSC |
29.14 |
30.13 |
0.99 |
| 13 |
Shelley, Sophia M (13)
|
PVRY |
30.40 |
30.85 |
0.45 |
| 14 |
McFarland, Lillian M (13)
|
PVRY |
31.29 |
31.22 |
-0.07 |
| 15 |
Shelley, Ella G (13)
|
PVRY |
32.28 |
31.35 |
-0.93 |
| 16 |
Semanchik, Margaret C (14)
|
PVRY |
32.19 |
31.53 |
-0.66 |
| 17 |
Quaresima, Gina M (15)
|
PVRY |
33.33 |
32.29 |
-1.04 |
| 18 |
Ferguson, Savannah M (14)
|
PVRY |
33.72 |
34.27 |
0.55 |
| 19 |
Rhodes, Shyla (14)
|
PVRY |
34.54 |
36.19 |
1.65 |
| 20 |
Gupta, Avni (14)
|
PVRY |
37.29 |
37.17 |
-0.12 |
| 21 |
Hager, Gabriella T (13)
|
PVRY |
39.31 |
38.95 |
-0.36 |
|
Snow, Sierra A (17)
|
MDFSC |
24.67 |
NS |
- |
|
Williams, Ava F (13)
|
PVRY |
28.70 |
NS |
- |