| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Filar, Logan (12)
|
WIDCY |
34.25 |
34.74 |
0.49 |
| 2 |
Soik, Mason (12)
|
WISPDY |
36.48 |
36.44 |
-0.04 |
| 3 |
Monfils, Jack R (11)
|
WIDCY |
36.98 |
38.83 |
1.85 |
| 4 |
Jannusch, Michael J (11)
|
WILAXY |
40.11 |
39.99 |
-0.12 |
| 5 |
Oshan, Arjun K (11)
|
WILAXY |
39.78 |
40.71 |
0.93 |
| 6 |
Hopp, Ben (11)
|
WISPDY |
40.25 |
41.58 |
1.33 |
| 7 |
Faust, Jacob (11)
|
WISPDY |
42.41 |
43.15 |
0.74 |
| 8 |
Klumb, Carson (11)
|
WIMYST |
44.73 |
44.36 |
-0.37 |
| 9 |
Filar, Luke (11)
|
WIDCY |
46.24 |
46.41 |
0.17 |
| 10 |
Raczek, Anthony R (11)
|
WISPDY |
43.72 |
46.57 |
2.85 |
| 11 |
Bicknase, Landon (12)
|
WISPDY |
47.22 |
47.50 |
0.28 |
| 12 |
Mischnick, Sage L (11)
|
WIWYNS |
49.34 |
48.18 |
-1.16 |
| 13 |
Mullikin, Zachariah J (11)
|
WILAXY |
51.55 |
50.87 |
-0.68 |
| 14 |
Haemmerle, Nathaniel (11)
|
WISPDY |
50.74 |
51.11 |
0.37 |
| 15 |
Bowling, Henry (11)
|
WISPDY |
1:13.87 |
57.50 |
-16.37 |
| 16 |
Lee, David P (12)
|
WISWC |
1:03.92 |
58.15 |
-5.77 |
|
Hoffman, Conner (12)
|
WISPDY |
|
DQ |
- |
|
Martens, Drake (12)
|
WISPDY |
1:29.38 |
DQ |
- |