| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Parson, Lucy J (10)
|
IABHCP |
32.42 |
30.75 |
-1.67 |
| 2 |
Klinkenborg, Madelyn M (10)
|
IABHCP |
33.17 |
32.32 |
-0.85 |
| 3 |
Engelkemier, Birdie J (10)
|
IAMUS |
36.70 |
34.25 |
-2.45 |
| 4 |
Pharr, Jaimee * (10)
|
IACRSP |
40.79 |
39.84 |
-0.95 |
| 5 |
Crist, Josie A (9)
|
IACRSP |
40.51 |
41.74 |
1.23 |
| 6 |
Hidlebaugh, Geneva S (10)
|
IABHCP |
40.44 |
41.99 |
1.55 |
| 7 |
Goldberg, Elise (9)
|
IACRSP |
41.99 |
43.82 |
1.83 |
| 8 |
Tudor, Mara E (9)
|
IABHCP |
43.06 |
45.93 |
2.87 |
| 8 |
Smith, Shiloh (9)
|
IACRSP |
44.13 |
45.93 |
1.80 |
| 10 |
Hodapp, Allie L (10)
|
IAMUS |
50.79 |
46.21 |
-4.58 |
| 11 |
Cochran, Kaylee E (9)
|
IAMUS |
1:00.20 |
48.53 |
-11.67 |
| 12 |
Pittman, Hannah M (9)
|
IAMUS |
50.54 |
50.32 |
-0.22 |
| 13 |
Packard, Zoe (9)
|
IACRSP |
50.61 |
51.14 |
0.53 |
| 14 |
Otten, Olivia O (9)
|
IACRSP |
54.09 |
56.64 |
2.55 |
|
Butler, Carly O (10)
|
IACRSP |
40.20 |
40.81 |
0.61 |