| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jovanovic, Mila (8)
|
MRVIP |
42.16 |
42.43 |
0.27 |
| 2 |
Hartnagel, Olivia R (7)
|
MRTVSC |
45.21 |
43.18 |
-2.03 |
| 3 |
Villagracia, Madison E (8)
|
MRTVSC |
50.06 |
44.03 |
-6.03 |
| 4 |
Rochlin, Vivienne (8)
|
MRTVSC |
48.44 |
44.22 |
-4.22 |
| 5 |
Bonn, Abigail (8)
|
MRHAA |
46.23 |
44.29 |
-1.94 |
| 6 |
Jukic, Mia A (7)
|
MRVIP |
45.08 |
44.33 |
-0.75 |
| 7 |
Lopez, Valeria (8)
|
MRVIP |
41.31 |
44.48 |
3.17 |
| 8 |
Wiedersum, Adelyn L (8)
|
MRTVSC |
40.46 |
44.82 |
4.36 |
| 9 |
Emmerich, Peyton M (7)
|
MRTVSC |
|
45.56 |
- |
| 10 |
Cullen, Moxie J (7)
|
MRWISC |
47.18 |
46.54 |
-0.64 |
| 11 |
Blumenauer, Lily A (7)
|
MRWISC |
49.37 |
46.56 |
-2.81 |
| 12 |
Rodgers, Addison R (8)
|
MRHAA |
1:01.45 |
47.40 |
-14.05 |
| 13 |
Kelly, Chloe F (8)
|
MRTVSC |
52.61 |
47.98 |
-4.63 |
| 14 |
Crennan, Payton J (7)
|
MRSSC |
58.16 |
49.84 |
-8.32 |
| 15 |
Lebiedzinska, Martyna M (8)
|
MRWISC |
50.27 |
50.29 |
0.02 |
| 16 |
Meliere, Emily R (8)
|
MRWISC |
57.84 |
52.61 |
-5.23 |
| 17 |
Keillor, Virginia A (7)
|
MRTVSC |
1:01.94 |
53.51 |
-8.43 |
| 18 |
Ghanmi, Soumya M (6)
|
MRSSC |
1:14.40 |
55.55 |
-18.85 |
| 19 |
DeHart, Addison (8)
|
MRHAA |
52.75 |
57.93 |
5.18 |
| 20 |
Birrer, Andi (8)
|
MRTS |
1:03.79 |
58.40 |
-5.39 |
| 21 |
Cerullo, Josephine S (8)
|
MRTVSC |
1:00.00 |
1:00.98 |
0.98 |
| 22 |
Johnson, Olivia F (8)
|
MRHAA |
59.15 |
1:03.97 |
4.82 |
| 23 |
Regan, McKenna K (8)
|
MRWISC |
1:04.26 |
1:06.24 |
1.98 |
| 24 |
Sclafani, Madison (7)
|
MRHAA |
1:19.77 |
1:07.35 |
-12.42 |
| 25 |
Chu, Jocelyn C (6)
|
MRTVSC |
1:06.05 |
1:08.06 |
2.01 |
| 26 |
Tishchenko, Sophia A (8)
|
MRSSC |
1:30.00 |
1:10.72 |
-19.28 |
| 27 |
Tomasello, Faith M (7)
|
MRHAA |
|
1:18.60 |
- |
| 28 |
Joyce, Adrianna G (6)
|
MRWISC |
1:48.13 |
1:20.84 |
-27.29 |
|
Flandrau, Harper E (6)
|
MRSSC |
1:00.00 |
NS |
- |