| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cogle, Siri A (12)
|
FGJDST |
40.72 |
39.05 |
-1.67 |
| 2 |
Patton, Mia K (12)
|
FGBEST |
39.95 |
39.86 |
-0.09 |
| 3 |
Baratta, Amanda M (12)
|
FGWOW |
39.66 |
41.24 |
1.58 |
| 4 |
Feeney, Lauren E (12)
|
FGUNAT |
44.34 |
42.56 |
-1.78 |
| 5 |
Guyett, Venice S (11)
|
FGECAC |
42.71 |
42.59 |
-0.12 |
| 6 |
Brennan, Madison R (12)
|
FGMART |
44.46 |
43.51 |
-0.95 |
| 7 |
Rubin, Makena (12)
|
FGWOW |
45.98 |
43.52 |
-2.46 |
| 8 |
Guzzo, Isabella V (11)
|
FGECAC |
43.37 |
43.54 |
0.17 |
| 9 |
Mulvaney, Catherine A (12)
|
FGMART |
54.58 |
43.65 |
-10.93 |
| 10 |
Wasielewski, Olivia L (11)
|
FGMART |
46.48 |
44.11 |
-2.37 |
| 11 |
Hidalgo Cadet, Maripaz (12)
|
FGJDST |
43.65 |
44.61 |
0.96 |
| 12 |
Urtula, Arabella C (11)
|
FGECAC |
45.87 |
45.96 |
0.09 |
| 13 |
Smart, Anabelle H (12)
|
FGMART |
|
47.16 |
- |
| 14 |
Pericchi, Karah M (11)
|
FGMART |
|
50.39 |
- |
| 15 |
Lord, Emory F (11)
|
FGMART |
55.83 |
50.53 |
-5.30 |
| 16 |
Rogers, Kathryn G (11)
|
FGMART |
51.73 |
50.85 |
-0.88 |
| 17 |
Keller, Molly (11)
|
FGJDST |
58.32 |
55.82 |
-2.50 |
|
Battjer, Lila C (11)
|
FGMART |
|
DQ |
- |
|
Tribou, Miriam E (11)
|
FGJDST |
51.19 |
NS |
- |