| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Berke, Jared I (10)
|
FGPCS |
41.96 |
42.74 |
0.78 |
| 2 |
Rendon, Adrian O (10)
|
FGMWA |
40.23 |
43.25 |
3.02 |
| 3 |
Caponera, Caleb T (9)
|
FGSAS |
44.79 |
44.54 |
-0.25 |
| 4 |
Ella, Daniel G (10)
|
FGSUN |
47.86 |
44.96 |
-2.90 |
| 5 |
Hall, Kai G (10)
|
FGWOW |
47.53 |
45.16 |
-2.37 |
| 6 |
Harries, Ryan T (10)
|
FGSOFL |
44.47 |
45.50 |
1.03 |
| 7 |
Vuong, Duy X (10)
|
FGNPB |
46.04 |
46.24 |
0.20 |
| 8 |
Kolobov, Tikhon (10)
|
FGNMST |
47.03 |
46.50 |
-0.53 |
| 9 |
Linares, Jesus D (10)
|
FGMACM |
48.12 |
46.75 |
-1.37 |
| 10 |
Aguila, Jorge E (10)
|
FGMWA |
45.35 |
46.93 |
1.58 |
| 11 |
Kotz-Agramonte, Jake I (10)
|
FGRANS |
42.95 |
47.00 |
4.05 |
| 12 |
Park, Brandon (10)
|
FGMWA |
48.97 |
47.22 |
-1.75 |
| 12 |
Milian, Jason (10)
|
FGBMHG |
46.69 |
47.22 |
0.53 |
| 14 |
Craine, Makai L (10)
|
FGJDST |
46.29 |
47.49 |
1.20 |
| 15 |
Felix De La Rosa, Wander A (10)
|
FGUN-3 |
48.98 |
47.53 |
-1.45 |
| 16 |
Menacho, Becket (10)
|
FGPOMP |
48.42 |
48.11 |
-0.31 |
| 17 |
Dipaolo, Bradley L (10)
|
FGCSSC |
47.59 |
48.40 |
0.81 |
| 18 |
Spiller, Jonah D (9)
|
FGHAT |
48.30 |
50.05 |
1.75 |
| 19 |
Woodard, Slater A (10)
|
FGJDST |
42.32 |
50.28 |
7.96 |
| 20 |
Fothergill, Liam (9)
|
FGMWA |
48.24 |
50.33 |
2.09 |
|
Vallmitjana, Juan (10)
|
FGSOFL |
44.25 |
NS |
- |