| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Petricevic, Madison (11)
|
MRWAC |
50.00 |
40.07 |
-9.93 |
| 2 |
Moore, Korianne M (12)
|
MRYBAR |
39.52 |
40.19 |
0.67 |
| 3 |
Labita, Julia (11)
|
MRNYSA |
39.80 |
40.24 |
0.44 |
| 4 |
Smith, Catherine M (12)
|
MRNYSA |
40.17 |
40.53 |
0.36 |
| 5 |
Donaldson, Savannah A (12)
|
MRYBAR |
41.69 |
42.60 |
0.91 |
| 6 |
Tirro, Theresa (12)
|
MRWAC |
44.31 |
43.44 |
-0.87 |
| 7 |
Armstrong, Claire M (12)
|
MRMWSC |
41.91 |
44.17 |
2.26 |
| 8 |
Stylianou, Helena (12)
|
MRWAC |
50.00 |
44.41 |
-5.59 |
| 9 |
Beezer, Kristina G (11)
|
MRMWSC |
44.71 |
44.43 |
-0.28 |
| 10 |
Lombardo, Jade (11)
|
MRWAC |
50.21 |
45.38 |
-4.83 |
| 11 |
Lussen, Katherine T (12)
|
MRNYSA |
49.25 |
46.10 |
-3.15 |
| 12 |
Klein, Lily (11)
|
MRNYSA |
53.56 |
48.01 |
-5.55 |
| 13 |
Guglielmello, Sophia (11)
|
MRMWSC |
48.74 |
49.60 |
0.86 |
| 14 |
Camo, Madeline N (11)
|
MRNYSA |
47.68 |
50.08 |
2.40 |
| 15 |
Jackman, Anayah M (12)
|
MRYBAR |
54.09 |
52.52 |
-1.57 |
| 16 |
Furman, Alyshea T (11)
|
MRYBAR |
1:12.43 |
52.84 |
-19.59 |
| 17 |
Brooks, Meadow M (11)
|
MRUNAT |
1:02.48 |
53.07 |
-9.41 |
| 18 |
Donovan, Mary (11)
|
MRNYSA |
|
1:01.27 |
- |
|
Kuns, Grace E (12)
|
MRYBAR |
56.02 |
DQ |
- |