| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yu, Charles R (11)
|
GU300 |
32.58 |
31.17 |
-1.41 |
| 2 |
Romero, Sebastian N (12)
|
GUESCA |
33.70 |
33.81 |
0.11 |
| 3 |
Suarez, Cruz L (11)
|
GUVSST |
33.80 |
34.04 |
0.24 |
| 4 |
Wang, Jason H (11)
|
GUKATY |
39.36 |
34.09 |
-5.27 |
| 5 |
Osode, Ifechukwude M (12)
|
GUKATY |
31.02 |
34.51 |
3.49 |
| 6 |
Yang, David K (12)
|
GUSSAN |
35.79 |
35.39 |
-0.40 |
| 7 |
Picen, Gianluca J (12)
|
GUESCA |
35.35 |
35.55 |
0.20 |
| 8 |
Rhoe-Yamaguchi, Edward T (11)
|
GUBATS |
33.88 |
35.72 |
1.84 |
| 9 |
Braband, Brendon P (11)
|
GUPACK |
37.95 |
35.86 |
-2.09 |
| 9 |
Al-Ramahi, Elias (11)
|
GURICE |
36.41 |
35.86 |
-0.55 |
| 11 |
Abril, Cole M (11)
|
GUSSAN |
36.48 |
36.16 |
-0.32 |
| 12 |
Carlson, Andrew J (11)
|
GUSSAN |
36.70 |
36.54 |
-0.16 |
| 13 |
Serpa, Arnaldo G (12)
|
GUESCA |
|
36.96 |
- |
| 14 |
Nguyen, Tom T (11)
|
GUESCA |
38.03 |
37.59 |
-0.44 |
| 15 |
Kim, Heesoo (12)
|
GUKATY |
40.01 |
37.71 |
-2.30 |
| 16 |
Cassens, Trenton C (12)
|
GUPACK |
40.66 |
37.83 |
-2.83 |
| 17 |
Figueredo-Weiser, Lukas (11)
|
GUVSST |
37.89 |
37.85 |
-0.04 |
| 18 |
Rainusso, Luca (12)
|
GURICE |
39.91 |
38.95 |
-0.96 |
| 19 |
Lam, William T (12)
|
GUESCA |
34.29 |
38.97 |
4.68 |
| 20 |
Brenes-Quesada, Javier (12)
|
GUPFL |
35.81 |
39.40 |
3.59 |
| 21 |
Zhao, Johnathan M (11)
|
GUPACK |
41.63 |
40.41 |
-1.22 |
| 22 |
Goebel, TaylorLee K (12)
|
GUPACK |
41.42 |
40.56 |
-0.86 |
| 23 |
Jo, Eunseok (12)
|
GUKATY |
47.47 |
41.20 |
-6.27 |
| 24 |
Cheng, Xu N (12)
|
GUKATY |
38.49 |
41.63 |
3.14 |
| 25 |
Cardenas, Reynaldo (11)
|
GUNOCH |
36.77 |
41.96 |
5.19 |
| 26 |
Gee, Christopher T (11)
|
GU300 |
|
42.78 |
- |
| 27 |
Combs, Elijah E (11)
|
GUPACK |
52.68 |
50.22 |
-2.46 |
| 28 |
Wang, Xunyang (11)
|
GUKATY |
|
56.64 |
- |