| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
La Pietra, Wendy G (6)
|
FGAZFL |
50.24 |
42.67 |
-7.57 |
| 2 |
Mirones, Lilliana M (8)
|
FGSUN |
45.78 |
43.77 |
-2.01 |
| 3 |
Rilo, Athena (8)
|
FGSUN |
43.87 |
44.86 |
0.99 |
| 4 |
Evans, Shelby O (8)
|
FGSOFL |
44.02 |
45.64 |
1.62 |
| 5 |
Royalty, Cherill L (7)
|
FGSOFL |
48.08 |
45.83 |
-2.25 |
| 6 |
Milevska, Eva (7)
|
FGAZFL |
47.03 |
46.12 |
-0.91 |
| 7 |
Martin, Gianna S (8)
|
FGSOFL |
1:02.04 |
46.82 |
-15.22 |
| 8 |
Jorgensen, Kierstyn E (8)
|
FGSUN |
53.84 |
47.99 |
-5.85 |
| 9 |
Ordonez, Valentina A (8)
|
FGTSA |
|
48.86 |
- |
| 10 |
Evans, Jordan C (7)
|
FGSOFL |
49.62 |
49.54 |
-0.08 |
| 11 |
Robinson, Aanya M (8)
|
FGSOFL |
53.70 |
51.86 |
-1.84 |
| 12 |
Zheng, Elaine L (8)
|
FGAZFL |
50.54 |
52.79 |
2.25 |
| 13 |
Jean-Baptiste, Daneika (7)
|
FGSUN |
|
52.97 |
- |
| 14 |
Poitier, Amyrah (8)
|
FGUN-T |
1:00.68 |
53.25 |
-7.43 |
| 15 |
El Chantiry, Isla M (7)
|
FGCSSC |
56.15 |
54.78 |
-1.37 |
| 16 |
Valdiviezo, Joanna R (7)
|
FGSF |
|
55.19 |
- |
| 17 |
Rodriguez Walker, Vanessa L (8)
|
FGCSSC |
57.70 |
55.42 |
-2.28 |
| 18 |
Svensson, Freja I (8)
|
FGSFTL |
|
55.72 |
- |
| 19 |
Velez, Camila S (8)
|
FGTSA |
|
58.45 |
- |
| 20 |
Sandoval, Maria D (7)
|
FGTSA |
|
1:00.24 |
- |
| 21 |
Marmolejo, Sara S (8)
|
FGSUN |
|
1:05.48 |
- |
| 22 |
Jorge, Kayla (8)
|
FGCSSC |
1:05.69 |
1:05.54 |
-0.15 |
| 23 |
Troupe, Azaria (7)
|
FGTSA |
|
1:09.51 |
- |
|
Melius, Angelina M (8)
|
FGHAFL |
57.14 |
DQ |
- |
|
St. Hilaire, Zahnajai (8)
|
FGSFTL |
49.59 |
NS |
- |