| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gonio, Margaret L (13)
|
FGSFTL |
30.83 |
28.99 |
-1.84 |
| 2 |
Schlicht, Isabel O (13)
|
FGYSF |
30.07 |
29.11 |
-0.96 |
| 3 |
Saballos, Fabiola (14)
|
FGPST |
30.69 |
29.38 |
-1.31 |
| 4 |
Colado, Sarah (14)
|
FGTSA |
30.14 |
29.70 |
-0.44 |
| 5 |
Dedon, Taylor A (13)
|
FGHAFL |
31.05 |
29.74 |
-1.31 |
| 6 |
Lissa, Gabriella D (13)
|
FGSOFL |
30.46 |
30.07 |
-0.39 |
| 7 |
Kuncas, Holly L (14)
|
FGSFTL |
30.54 |
30.14 |
-0.40 |
| 8 |
Peacey, Ava K (13)
|
FGSFTL |
30.13 |
30.21 |
0.08 |
| 9 |
Mizrachi, Elleyah R (13)
|
FGPST |
32.56 |
31.00 |
-1.56 |
| 10 |
Blanchard, Tamalia (13)
|
FGSF |
32.50 |
31.20 |
-1.30 |
| 11 |
Fahnestock, Isabella G (14)
|
FGCSSC |
32.18 |
31.80 |
-0.38 |
| 12 |
Gonzalez, Breanna (14)
|
FGSUN |
31.13 |
32.35 |
1.22 |
| 13 |
Parsotan, Nadia A (13)
|
FGPST |
32.85 |
33.06 |
0.21 |
| 14 |
Blanchard, Makaila (13)
|
FGSF |
32.90 |
33.07 |
0.17 |
| 15 |
Bailey, Justine L (14)
|
FGCCPR |
|
34.10 |
- |
| 16 |
Ramirez, Sofia I (14)
|
FGAZFL |
38.59 |
34.28 |
-4.31 |
| 17 |
Reddick, Shaniyah L (14)
|
FGSFTL |
|
34.79 |
- |
| 18 |
Feudo, Jillian G (13)
|
FGPST |
38.50 |
38.31 |
-0.19 |
|
Consuegra, Kaitlyn C (13)
|
FGSOFL |
30.18 |
NS |
- |