| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lowery, Brian D (13)
|
ILVY |
25.43 |
25.23 |
-0.20 |
| 2 |
Graham, Matteo R (13)
|
ILDCST |
26.53 |
26.94 |
0.41 |
| 3 |
Gilbert, Gavin L (14)
|
ILFVFY |
27.89 |
27.23 |
-0.66 |
| 4 |
Campbell, Timothy I (14)
|
ILFVFY |
27.71 |
27.48 |
-0.23 |
| 4 |
Harrelson, Evan R (14)
|
ILDCST |
27.68 |
27.48 |
-0.20 |
| 6 |
Skrzypek, Alexander E (13)
|
ILDCST |
27.95 |
28.29 |
0.34 |
| 7 |
Uhlenhopp, Mattson H (14)
|
ILFVFY |
29.23 |
28.79 |
-0.44 |
| 8 |
Svarc, Derek (14)
|
ILRAYS |
|
28.83 |
- |
| 9 |
Harrelson, Sean A (13)
|
ILDCST |
29.92 |
29.67 |
-0.25 |
| 10 |
Brunson, Kairo (14)
|
ILRAYS |
29.59 |
30.87 |
1.28 |
| 11 |
Smith, Cameron W (14)
|
ILFVFY |
31.75 |
31.23 |
-0.52 |
| 12 |
Crawford, Owen (13)
|
ILRAYS |
|
35.70 |
- |
| 13 |
Brunson, Deangello (13)
|
ILRAYS |
51.36 |
43.82 |
-7.54 |