| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rychlik, Kyle A (14)
|
ILVHST |
56.17 |
52.73 |
-3.44 |
| 2 |
Miles, Cale K (14)
|
ILUNMA |
55.72 |
53.06 |
-2.66 |
| 3 |
Maere, Skylar T (13)
|
ILMARL |
55.41 |
53.36 |
-2.05 |
| 4 |
Rayford, Warren K (14)
|
ILUN |
55.47 |
54.29 |
-1.18 |
| 5 |
Isaacs, Paul S (14)
|
ILMARL |
57.61 |
54.59 |
-3.02 |
| 6 |
Helfrich, Gage R (12)
|
ILDCST |
1:00.01 |
55.95 |
-4.06 |
| 7 |
Dolan, Matt B (14)
|
ILMAC |
1:02.00 |
57.18 |
-4.82 |
| 8 |
Axelson, Jakob J (14)
|
ILVHST |
1:00.55 |
58.82 |
-1.73 |
| 9 |
Sullivan, Kevin G (13)
|
ILDCST |
1:01.47 |
59.22 |
-2.25 |
| 10 |
Skrzypek, Alexander E (13)
|
ILDCST |
1:01.63 |
1:01.46 |
-0.17 |
| 11 |
De La Cruz, Samuel S (13)
|
ILDCST |
1:01.88 |
1:01.69 |
-0.19 |
| 12 |
Uhlenhopp, Mattson H (13)
|
ILFVFY |
1:07.62 |
1:02.85 |
-4.77 |
| 13 |
Breese, Aidan J (13)
|
ILRMSC |
1:12.05 |
1:07.00 |
-5.05 |
| 14 |
Gilbert, Gavin L (14)
|
ILFVFY |
1:06.46 |
1:07.17 |
0.71 |
| 16 |
Markou, Kosta (12)
|
ILVHST |
|
1:07.77 |
- |
| 17 |
Hartman, Dean (13)
|
ILFAST |
1:13.59 |
1:09.14 |
-4.45 |
| 18 |
Murray, James C (13)
|
ILRMSC |
1:14.44 |
1:09.26 |
-5.18 |
| 19 |
Helm, Colin J (13)
|
ILFAST |
1:13.84 |
1:15.79 |
1.95 |
| 20 |
Hallom, Demari J (13)
|
ILFAST |
|
1:36.54 |
- |