| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nieckula, Ariyana E (11)
|
ILFVFY |
36.79 |
35.45 |
-1.34 |
| 2 |
Brereton, Penelope J (11)
|
ILSAGE |
36.12 |
35.86 |
-0.26 |
| 3 |
Esparza, Ruby K (11)
|
ILRAYS |
38.16 |
38.65 |
0.49 |
| 4 |
Tomaszewski, Kaitlyn G (11)
|
ILSAGE |
38.73 |
38.94 |
0.21 |
| 5 |
Rudsinski, Emely G (12)
|
ILSAGE |
38.59 |
39.04 |
0.45 |
| 6 |
DeSpain, Mikenna L (12)
|
ILDCST |
38.51 |
39.65 |
1.14 |
| 7 |
Cork, Sophia K (12)
|
ILDCST |
39.32 |
40.34 |
1.02 |
| 8 |
Vollmer, Emily M (11)
|
ILFVFY |
43.81 |
41.01 |
-2.80 |
| 9 |
Miller, Lily E (11)
|
ILOTTY |
41.16 |
41.08 |
-0.08 |
| 10 |
Gillibrand, Sage E (11)
|
ILFVFY |
42.33 |
42.14 |
-0.19 |
| 11 |
Haskell, Mirella A (12)
|
ILVY |
41.99 |
42.21 |
0.22 |
| 12 |
Ziegler, Abigail J (11)
|
ILFVFY |
42.79 |
42.85 |
0.06 |
| 13 |
Bloyd, Emma A (11)
|
ILDCST |
43.08 |
43.27 |
0.19 |
| 14 |
Zimmerman, Natalie L (11)
|
ILSAGE |
43.70 |
44.17 |
0.47 |
| 15 |
Kocjan, Alissa S (12)
|
ILDCST |
46.65 |
44.63 |
-2.02 |
| 16 |
Fermin, Ariana * (12)
|
ILSAGE |
46.90 |
46.17 |
-0.73 |
| 17 |
Maces, Melecia L (12)
|
ILFVFY |
48.48 |
46.92 |
-1.56 |
| 18 |
Gobert, Alexandria K (12)
|
ILRAYS |
49.40 |
48.00 |
-1.40 |
| 19 |
Rudnytsky, Alexandra (12)
|
ILPALA |
53.35 |
49.57 |
-3.78 |
| 20 |
Thompson, Susannah J (11)
|
ILPALA |
53.93 |
50.76 |
-3.17 |
| 21 |
Urbates, Ella K (11)
|
ILSAGE |
49.52 |
51.40 |
1.88 |
| 22 |
Burke, Makena J (11)
|
ILOTTY |
52.21 |
52.42 |
0.21 |
| 23 |
Brewer, Lainey E (12)
|
ILFVFY |
57.25 |
54.71 |
-2.54 |
| 24 |
Jordan, Bernadette M (11)
|
ILFVFY |
58.13 |
55.52 |
-2.61 |
| 25 |
Chafai, Sarah (11)
|
ILPALA |
57.23 |
56.34 |
-0.89 |
|
Vander Bleek, Samantha G (10)
|
ILDCST |
|
DQ |
- |
|
Noble, Trinity * (12)
|
ILRAYS |
|
DQ |
- |
|
Fultz, Addison M (12)
|
ILOTTY |
48.07 |
NS |
- |