| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Teng, Victor T (14)
|
NIISWM |
2:55.72 |
2:57.51 |
1.79 |
| 2 |
Busch, Nolan S (15)
|
NIOLSC |
|
2:57.98 |
- |
| 3 |
Glaubitz, Flaminio S (15)
|
NIISWM |
3:04.13 |
2:58.99 |
-5.14 |
| 4 |
McMahon, Ryan M (15)
|
NICSCI |
3:00.30 |
3:00.77 |
0.47 |
| 5 |
Lister, Gwenyth E (12)
|
NISYCH |
3:06.47 |
3:04.68 |
-1.79 |
| 6 |
Thorne, Lauren E (18)
|
NIJETS |
2:59.29 |
3:05.83 |
6.54 |
| 7 |
Eckman, Rebekah L (15)
|
ADALBS |
3:05.61 |
3:10.14 |
4.53 |
| 8 |
Mueller, Christopher J (14)
|
NISDSC |
3:07.66 |
3:11.31 |
3.65 |
| 9 |
Ottaviano, Catherine E (14)
|
NICSCI |
3:12.56 |
3:15.81 |
3.25 |
| 10 |
Lawton, Sydney L (15)
|
NIMETS |
3:19.25 |
3:19.80 |
0.55 |
| 11 |
Bebla, Sofia G (13)
|
NISYCH |
3:18.49 |
3:20.83 |
2.34 |
| 12 |
Gellert, Delaney E (13)
|
NIJETS |
3:27.92 |
3:29.28 |
1.36 |
| 13 |
Mertha, Sophie R (13)
|
NIISWM |
3:36.26 |
3:40.95 |
4.69 |
| 14 |
de Ruijter, Domenique (13)
|
NIJETS |
3:43.80 |
3:41.09 |
-2.71 |
| 15 |
Goodreau, Josephine M (12)
|
NIISWM |
|
3:50.13 |
- |
| 16 |
Urazgildiiev, Renat I (14)
|
NIISWM |
3:52.74 |
4:03.90 |
11.16 |
| 17 |
Sparks, Julia M (11)
|
NICSCI |
|
4:17.24 |
- |
|
Wong, Michael (13)
|
ADALBS |
3:42.97 |
NS |
- |
|
Depew, Meghan C (16)
|
ADALBS |
3:15.82 |
NS |
- |