| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Arreguin, Ayleen (12)
|
GUHCAP |
30.42 |
31.67 |
1.25 |
| 2 |
Andrade, Mia A (11)
|
GUSSS |
33.15 |
32.00 |
-1.15 |
| 3 |
Sliger, Madalynn (11)
|
GUTCAP |
31.62 |
32.89 |
1.27 |
| 4 |
Ojeda, Linnette A (12)
|
GUHCAP |
|
33.14 |
- |
| 5 |
Foster, Tyler G (11)
|
GUSSS |
39.62 |
33.48 |
-6.14 |
| 6 |
Medellin, Mackenzie K (11)
|
GUSSS |
34.82 |
33.86 |
-0.96 |
| 7 |
Garcia, Alayda I (12)
|
GUNOCH |
34.84 |
34.57 |
-0.27 |
| 8 |
Nguyen, Sariel Q (11)
|
GUSCAT |
38.52 |
35.06 |
-3.46 |
| 9 |
Pham, Haleigh T (12)
|
GUSSS |
34.95 |
35.07 |
0.12 |
| 10 |
Delasbour, Tatyana J (11)
|
GUHCAP |
38.93 |
35.13 |
-3.80 |
| 11 |
Massey, Jubilee G (11)
|
GUHCAP |
40.97 |
35.15 |
-5.82 |
| 12 |
Woodby, Addison N (11)
|
GUAQUA |
34.35 |
35.36 |
1.01 |
| 13 |
Garcia, Ava E (12)
|
GUNOCH |
34.18 |
35.52 |
1.34 |
| 14 |
Garcia, Leslie F (11)
|
GUAQUA |
34.51 |
37.90 |
3.39 |
| 15 |
Blacklock, Jiah E (11)
|
GUHCAP |
40.34 |
38.74 |
-1.60 |
| 16 |
Moad, Addyson L (11)
|
GUUN-S |
41.88 |
38.92 |
-2.96 |
| 17 |
Stokes, Abby (11)
|
GUTCAP |
40.20 |
39.21 |
-0.99 |
| 18 |
Torres, Avery (12)
|
GUTCAP |
38.23 |
39.51 |
1.28 |
| 19 |
Castro, Miaangelina (11)
|
GUHCAP |
39.80 |
40.42 |
0.62 |
| 20 |
Ford, Aven M (11)
|
GUHCAP |
42.53 |
41.93 |
-0.60 |
| 21 |
Scanlon, Kassidy (11)
|
GUTCAP |
45.95 |
45.92 |
-0.03 |
| 22 |
Juillerat, Hallie M (11)
|
GUSSS |
56.00 |
50.23 |
-5.77 |