| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morton, Madelyn J (14)
|
NTCOPS |
28.12 |
27.98 |
-0.14 |
| 2 |
Sizemore, Danica X (13)
|
NTMTRO |
28.09 |
28.38 |
0.29 |
| 3 |
Sundberg Clemente, Alba (14)
|
NTMTRO |
28.00 |
28.80 |
0.80 |
| 4 |
Morales, Michele (13)
|
NTCOR |
28.87 |
29.36 |
0.49 |
| 5 |
Liles, Natalie T (14)
|
NTMTRO |
31.56 |
29.46 |
-2.10 |
| 6 |
Schaefer, Grace A (13)
|
NTCOPS |
29.19 |
29.89 |
0.70 |
| 7 |
Krishna, Shriya (14)
|
NTMTRO |
30.81 |
29.98 |
-0.83 |
| 8 |
Rallapalli, Adithi L (14)
|
NTCOR |
29.41 |
30.05 |
0.64 |
| 9 |
Cady, Alyssandra R (13)
|
NTMTRO |
32.77 |
33.19 |
0.42 |
| 10 |
Waidler, Ruby M (13)
|
NTCOR |
30.92 |
33.48 |
2.56 |
| 11 |
Lawrence, Isabel A (14)
|
NTCOPS |
32.33 |
33.78 |
1.45 |
| 12 |
Dong, Rebecca (13)
|
NTMTRO |
34.12 |
33.93 |
-0.19 |
| 13 |
Spencer, Eden K (13)
|
NTCOR |
36.84 |
36.64 |
-0.20 |
|
Ciske, Grace A (14)
|
NTCOPS |
28.26 |
NS |
- |