| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fiore, Mariella S (7)
|
MAUNAT |
43.83 |
38.78 |
-5.05 |
| 2 |
McGlinchey, Simone I (7)
|
MAGPAC |
40.54 |
40.40 |
-0.14 |
| 3 |
Kenderdine, Skylar P (8)
|
MATWAC |
|
42.60 |
- |
| 4 |
Berry, Alyssa M (8)
|
MATWAC |
41.80 |
45.89 |
4.09 |
| 5 |
Protomastro, Catarina R (8)
|
NJTAC |
|
52.72 |
- |
| 6 |
Bagley, Ava (7)
|
MASAKA |
58.58 |
58.20 |
-0.38 |
| 7 |
Sheldon, Samantha M (8)
|
MAJRAY |
54.21 |
58.24 |
4.03 |
| 8 |
Armstrong, Elizabeth A (8)
|
MASAKA |
|
1:04.67 |
- |
| 9 |
Barber, Hannah N (8)
|
MAJRAY |
1:11.63 |
1:15.60 |
3.97 |
|
Rosen, Rhylee N (7)
|
MANSC |
|
NS |
- |
|
Tufano, Sofia A (8)
|
MATWAC |
46.69 |
NS |
- |