| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wilson, Christian (13)
|
NITWST |
28.17 |
27.14 |
-1.03 |
| 2 |
Sippel, Liam D (14)
|
NITWST |
29.70 |
27.63 |
-2.07 |
| 3 |
McCrone, Patrick (14)
|
NITWST |
31.18 |
27.72 |
-3.46 |
| 4 |
Kruglov, Maxim A (14)
|
NIUN-T |
31.31 |
28.43 |
-2.88 |
| 5 |
Sullivan, Sawyer T (13)
|
NIHAMB |
30.19 |
28.96 |
-1.23 |
| 6 |
DeMartinis, Ethan W (14)
|
NIMERC |
31.35 |
29.69 |
-1.66 |
| 7 |
Wangelin, Charlie L (14)
|
NITWST |
32.25 |
30.46 |
-1.79 |
| 8 |
Haak, Miles S (13)
|
NITWST |
33.67 |
31.55 |
-2.12 |
| 9 |
Santarsiero, Ryan J (13)
|
NICLSC |
|
32.43 |
- |
| 10 |
Staley, Nicholas R (13)
|
NIMERC |
35.81 |
32.75 |
-3.06 |
| 11 |
Sperry, Zachary J (13)
|
NITTSC |
32.86 |
33.44 |
0.58 |
| 12 |
Jensen, Stuart M (13)
|
NICLSC |
|
35.93 |
- |
| 13 |
Cole, Matthew D (13)
|
NICLSC |
|
36.71 |
- |
| 14 |
Betterelli, Tristan J (13)
|
NIVELO |
|
38.21 |
- |
| 15 |
Gaspar, Brandon T (14)
|
NITWST |
38.33 |
39.14 |
0.81 |
| 16 |
Kendall, Jack S (13)
|
NITTSC |
|
1:03.96 |
- |
|
Sullivan, Nicholas S (13)
|
NICLSC |
44.44 |
NS |
- |