| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walier, Nora E (7)
|
NITWST |
42.49 |
37.65 |
-4.84 |
| 2 |
Carducci, Elizabeth G (8)
|
NITWST |
42.74 |
38.31 |
-4.43 |
| 3 |
Miller, Emery S (8)
|
NIHAMB |
43.81 |
39.60 |
-4.21 |
| 4 |
Spino, Kadence E (8)
|
NITWST |
44.48 |
42.21 |
-2.27 |
| 5 |
Bruzgul, Vaughn S (8)
|
NITWST |
49.17 |
42.49 |
-6.68 |
| 6 |
Marcyan, Reese J (8)
|
NITTSC |
48.37 |
43.26 |
-5.11 |
| 7 |
Stancliff, Katherine J (8)
|
NITTSC |
|
44.33 |
- |
| 8 |
Hufnagel, Megan K (7)
|
NITTSC |
47.27 |
44.87 |
-2.40 |
| 9 |
Rider, Charlotte P (8)
|
NIFALC |
|
46.50 |
- |
| 10 |
Cook, Hannah R (8)
|
NIHAMB |
52.36 |
50.41 |
-1.95 |
| 11 |
Brownell, Grace M (7)
|
NISTAR |
|
56.48 |
- |
| 12 |
Pashler, Amelia D (8)
|
NITTSC |
1:13.19 |
58.17 |
-15.02 |
| 13 |
Regelski, Lucy R (8)
|
NISTAR |
|
1:00.40 |
- |
| 14 |
Adams, Elliana H (7)
|
NISTAR |
|
1:00.73 |
- |
| 15 |
Scarpace, Milania G (6)
|
NITTSC |
1:04.26 |
1:09.49 |
5.23 |
| 16 |
Pashler, Nora D (6)
|
NITTSC |
|
1:13.58 |
- |
|
Lyons, Josie M (6)
|
NITTSC |
|
NS |
- |