| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kern, Kendra M (16)
|
OHMCY |
1:04.90 |
1:03.53 |
-1.37 |
| 2 |
Cowell, Grace M (15)
|
OHKEY |
1:05.57 |
1:03.88 |
-1.69 |
| 3 |
Clark, McKenna M (15)
|
OHCFY |
1:06.34 |
1:05.73 |
-0.61 |
| 4 |
Kitchen, Logan E (17)
|
OHCFY |
1:05.04 |
1:06.33 |
1.29 |
| 5 |
Thomas, Gwyneth M (16)
|
OHSPY |
1:06.94 |
1:06.52 |
-0.42 |
| 6 |
Cox, Colleen M (17)
|
OHDCY |
1:06.43 |
1:07.19 |
0.76 |
| 7 |
Frasure, Logan R (16)
|
OHSIDY |
1:09.10 |
1:08.27 |
-0.83 |
| 8 |
Wyers, Alexandria Z (15)
|
OHCFY |
1:08.73 |
1:09.04 |
0.31 |
| 9 |
Swedlund, Katherine A (16)
|
OHKEY |
1:09.84 |
1:05.45 |
-4.39 |
| 10 |
Eng, Elizabeth C (17)
|
OHCFY |
1:11.32 |
1:06.69 |
-4.63 |
| 11 |
Eckert, Cassandra M (16)
|
OHCFY |
1:11.47 |
1:08.49 |
-2.98 |
| 12 |
Jernovics, Emma C (16)
|
OHCFY |
1:12.39 |
1:09.38 |
-3.01 |
| 13 |
Dietz, Taylor K (15)
|
OHSPY |
1:09.62 |
1:09.49 |
-0.13 |
| 14 |
Pope, Hanna C (15)
|
OHCFY |
1:09.28 |
1:09.62 |
0.34 |
| 15 |
Link, Alivia (17)
|
OHAMFY |
1:14.07 |
1:12.62 |
-1.45 |
| 16 |
Braunschneider, Olivia C (15)
|
OHCFY |
1:13.86 |
1:13.84 |
-0.02 |