| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Young, Julia T (10)
|
ADTAC |
37.15 |
36.47 |
-0.68 |
| 2 |
Johnston, Layla A (10)
|
ADGLFY |
38.13 |
36.61 |
-1.52 |
| 3 |
Basile, Isabella M (10)
|
ADGRGF |
37.89 |
37.65 |
-0.24 |
| 4 |
Racaza, Reyna S (10)
|
ADBSPA |
|
38.22 |
- |
| 5 |
Bradley, Brooke A (10)
|
ADBFIN |
42.09 |
39.97 |
-2.12 |
| 6 |
Saunders, Mya J (9)
|
ADGRGF |
43.40 |
41.07 |
-2.33 |
| 7 |
Ho, Sophie (10)
|
ADDELM |
43.20 |
41.12 |
-2.08 |
| 8 |
De Tuya, Elizabeth M (10)
|
ADDELM |
42.55 |
41.31 |
-1.24 |
| 9 |
Allinson, Mae L (10)
|
ADDELM |
41.66 |
41.37 |
-0.29 |
| 10 |
Robles, Isabella M (10)
|
ADDELM |
42.50 |
41.69 |
-0.81 |
| 11 |
Miron, Sofia L (10)
|
ADBFIN |
42.06 |
41.90 |
-0.16 |
| 12 |
Candee, Isabella K (10)
|
NIUN |
42.17 |
41.99 |
-0.18 |
| 13 |
Relyea, Cadence G (9)
|
ADDELM |
44.35 |
42.94 |
-1.41 |
| 14 |
Bette, Julia A (9)
|
ADBFIN |
48.64 |
43.53 |
-5.11 |
| 15 |
Svare, Nuala S (10)
|
ADDELM |
50.15 |
45.63 |
-4.52 |
| 16 |
Chen, Serena (10)
|
ADBFIN |
48.51 |
46.43 |
-2.08 |
| 17 |
Murray, Aidan M (10)
|
ADCPP |
50.52 |
51.60 |
1.08 |
| 18 |
Tamayo, Madelyn C (10)
|
ADBFIN |
55.72 |
52.67 |
-3.05 |
| 19 |
Murphy, Abigail E (9)
|
ADBFIN |
1:01.20 |
1:01.09 |
-0.11 |
|
Durfee, Emily G (9)
|
ADGLFY |
44.64 |
DQ |
- |
|
Odorisio, Margaret G (10)
|
ADBFIN |
50.28 |
NS |
- |
|
McManus, Olivia M (10)
|
NIUN |
37.39 |
NS |
- |