| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fegley, Brooke A (18)
|
LAYNWL |
27.46 |
28.69 |
1.23 |
| 2 |
Thompson, Talia E (17)
|
NTTRAC |
29.58 |
29.86 |
0.28 |
| 3 |
Kolb, Diana E (19)
|
GUAMBU |
30.01 |
30.73 |
0.72 |
| 4 |
Squyres, Ava N (15)
|
NTTRAC |
34.75 |
32.75 |
-2.00 |
| 5 |
Courtney, Natalie L (15)
|
GUAMBU |
32.74 |
32.96 |
0.22 |
| 6 |
Deshotel, Madison N (16)
|
NTTRAC |
|
34.58 |
- |
| 7 |
Armstrong, Jordan D (17)
|
GUAMBU |
33.88 |
34.97 |
1.09 |
| 8 |
Romanski, Isabella G (15)
|
LAYNWL |
36.16 |
35.23 |
-0.93 |
| 9 |
Nieberding, Vivyan E (15)
|
GUAMBU |
38.27 |
36.17 |
-2.10 |
| 10 |
Wilson, Madison C (17)
|
NTTRAC |
|
36.83 |
- |
| 11 |
Stout, Taylor G (16)
|
NTMTRO |
36.40 |
37.56 |
1.16 |
| 12 |
Vahovius, Mary-Catherine (17)
|
NTMTRO |
|
39.75 |
- |
|
Lemmert, Olivia L (15)
|
NTTRAC |
32.11 |
DQ |
- |