| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vo, Phu T (9)
|
NTLIFE |
|
37.52 |
- |
| 2 |
Tracey, Iorwerth S (10)
|
LAYNWL |
38.59 |
40.35 |
1.76 |
| 3 |
Everson, Andrew G (9)
|
LAYNWL |
43.63 |
40.62 |
-3.01 |
| 4 |
Knighton, Jase P (9)
|
LAYNWL |
41.61 |
41.30 |
-0.31 |
| 5 |
Martine, Carter E (10)
|
NTTRAC |
|
41.67 |
- |
| 6 |
Purnama-Yang, Quentin H (10)
|
NTLIFE |
41.38 |
41.92 |
0.54 |
| 7 |
Messina, Joshua M (10)
|
NTLIFE |
|
42.65 |
- |
| 8 |
Martinez, Breyden C (9)
|
NTTRAC |
|
42.83 |
- |
| 9 |
McGuirk, Elliot R (10)
|
LAYNWL |
44.46 |
43.09 |
-1.37 |
| 10 |
Cloutet, William E (9)
|
LAYNWL |
45.82 |
44.04 |
-1.78 |
| 11 |
Purnama-Yang, Maxwell V (10)
|
NTLIFE |
49.86 |
44.80 |
-5.06 |
| 12 |
Jeter, Mason L (10)
|
NTTRAC |
|
48.23 |
- |
| 13 |
Johnson, Griffen E (9)
|
NTMTRO |
|
49.71 |
- |
| 14 |
Fernandez, Caleb E (10)
|
NTTRAC |
|
56.97 |
- |
| 15 |
Jackson, Peyton P (9)
|
NTMTRO |
|
1:03.09 |
- |