| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Alward, Luke J (12)
|
MIFLY |
32.67 |
31.89 |
-0.78 |
| 2 |
Hofmann, Drew R (12)
|
MIBWO |
32.03 |
33.26 |
1.23 |
| 3 |
O'Grady, Sawyer W (12)
|
MIRAYS |
34.68 |
36.54 |
1.86 |
| 4 |
Luehmann, Niklas V (12)
|
MIRAYS |
38.93 |
37.09 |
-1.84 |
| 5 |
McALpine, Lincoln F (11)
|
MIRAYS |
36.61 |
37.32 |
0.71 |
| 6 |
Davis, Andrew C (12)
|
MINOW |
38.40 |
38.47 |
0.07 |
| 7 |
Yarema, Luke D (11)
|
MIBBD |
38.17 |
38.88 |
0.71 |
| 8 |
DeBenedictis, Andrew R (12)
|
MINOW |
38.90 |
39.60 |
0.70 |
| 9 |
Louis, John M (12)
|
MINOW |
47.71 |
43.11 |
-4.60 |
| 10 |
Scott, Hayden (12)
|
MIYCAD |
40.53 |
44.22 |
3.69 |
| 11 |
Chaparro, Nicolas M (11)
|
MINOW |
45.67 |
44.42 |
-1.25 |
| 12 |
Rutterbush, Jared (12)
|
MIYCAD |
47.36 |
49.00 |
1.64 |
| 13 |
Roggenbuck, Nicholas J (11)
|
MINOW |
47.57 |
50.21 |
2.64 |
| 14 |
Youkoung, Julian C (11)
|
6SEA |
|
56.14 |
- |
|
Ruiz, Stephen (11)
|
MIBWO |
36.60 |
NS |
- |
|
Johnson, Lucas M (11)
|
MIBWO |
47.59 |
NS |
- |
|
Baldwin, Sena W (11)
|
MIBWO |
38.34 |
NS |
- |