| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hasis, Nicole (10)
|
MAWAY |
52.83 |
47.83 |
-5.00 |
| 1 |
Kifer, Hannah (10)
|
MASCAY |
|
48.31 |
- |
| 2 |
Hickey, Lillian G (10)
|
MATRI |
55.32 |
48.40 |
-6.92 |
| 3 |
MacNeely, Camryn J (10)
|
MANLAC |
|
48.95 |
- |
| 4 |
Karasinski, Gabriella N (9)
|
MAUN-8 |
|
52.17 |
- |
| 5 |
Stillwagon, Ella J (10)
|
AMGCY |
|
52.37 |
- |
| 6 |
Gounder, Kavya R (10)
|
MAGSA |
|
53.02 |
- |
| 7 |
Turner, Hannah M (10)
|
MAEPAC |
56.73 |
54.53 |
-2.20 |
| 8 |
Gyenes, Madison E (10)
|
MACYA |
|
55.02 |
- |
| 9 |
Torelli, Samantha C (9)
|
MATRI |
|
58.31 |
- |
| 10 |
Persun, Kiley N (9)
|
MAWASC |
1:08.95 |
1:01.52 |
-7.43 |
| 11 |
Kurpeikis, Olivia G (9)
|
MANLAC |
|
1:05.07 |
- |
| 12 |
Campbell, Kaitlyn M (10)
|
MASCAY |
|
1:05.98 |
- |
|
Messmer, Gabriella B (10)
|
MAGSA |
|
DQ |
- |
|
Boughanmi, Tasneem M (9)
|
MAEPAC |
|
NS |
- |
|
Frascella, Juliana R (9)
|
MATRI |
|
NS |
- |