| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walborn, Mabel R (10)
|
MAUN-5 |
41.19 |
39.13 |
-2.06 |
| 1 |
Hosley, Gabriella G (10)
|
MAUN-6 |
42.27 |
41.07 |
-1.20 |
| 1 |
Fuller, Claire M (10)
|
MANLAC |
51.53 |
49.42 |
-2.11 |
| 2 |
Giryanskaya, Margarita (10)
|
MANLAC |
49.58 |
49.48 |
-0.10 |
| 3 |
Case, Teagan L (10)
|
MANLAC |
|
50.13 |
- |
| 4 |
Trego, Natalie K (9)
|
MANLAC |
58.55 |
56.17 |
-2.38 |
| 5 |
Coakley, Sloane V (10)
|
MAUN-6 |
|
57.79 |
- |
| 6 |
Kraft, Clara R (9)
|
MANLAC |
1:05.74 |
58.24 |
-7.50 |
| 7 |
Huggins, Natalie V (9)
|
MANLAC |
1:04.07 |
58.70 |
-5.37 |
|
Masters, Katherine H (10)
|
MANLAC |
|
DQ |
- |
|
Mattiacci, Deanna M (10)
|
MAPENN |
|
DQ |
- |
|
Prentice, Samantha K (9)
|
MAPENN |
|
DQ |
- |