| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pfaff, Rabea K (14)
|
MADST |
2:19.47 |
2:27.29 |
7.82 |
| 2 |
Menzer, Emma G (14)
|
MADST |
2:31.65 |
2:35.30 |
3.65 |
| 3 |
Parker, Aleva C (16)
|
MAOCCC |
2:42.19 |
2:41.46 |
-0.73 |
| 4 |
Wilson, Logan A (11)
|
MADST |
2:48.95 |
2:42.27 |
-6.68 |
| 5 |
Melcher, Blair T (17)
|
MAGNBC |
2:34.56 |
2:43.70 |
9.14 |
| 6 |
Dickson, Heidi R (14)
|
MADST |
2:34.81 |
2:46.02 |
11.21 |
| 7 |
Warner, Bella N (13)
|
MADST |
2:45.49 |
2:47.84 |
2.35 |
| 8 |
Weaverling, Catherine A (12)
|
MADST |
2:52.77 |
2:49.67 |
-3.10 |
| 9 |
Dryden, Stephanie M (13)
|
MAGNBC |
2:52.05 |
2:52.22 |
0.17 |
| 10 |
Ryan, Lenya H (11)
|
MADST |
3:01.90 |
2:53.06 |
-8.84 |
| 11 |
Horowitz, Samantha E (17)
|
MAOCCC |
|
3:02.50 |
- |
| 12 |
Petrovia, Arianna M (14)
|
MANRG |
2:44.47 |
3:03.18 |
18.71 |
| 13 |
Bargfrede, Deven M (14)
|
MAWAVE |
|
3:05.28 |
- |
| 14 |
Feinberg, Lindsay T (11)
|
MAEAAC |
3:06.65 |
3:06.16 |
-0.49 |
| 15 |
Boettcher, Alexandria P (13)
|
MADST |
|
3:13.50 |
- |
| 16 |
Chilelli, Kathryn A (16)
|
MAJRAY |
3:24.48 |
3:33.86 |
9.38 |
| 17 |
Han, Kaylin (10)
|
MADST |
|
3:49.26 |
- |
|
Thomas, Avery L (13)
|
MADST |
|
NS |
- |
|
Belair, Emily G (9)
|
MADST |
|
NS |
- |
|
Kusters, Samantha R (15)
|
MANRG |
2:51.29 |
NS |
- |
|
Lebed, Michelle (14)
|
MANRG |
2:51.07 |
NS |
- |