| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Berkhof, Bryce A (15)
|
NIVICT |
24.28 |
24.50 |
0.22 |
| 2 |
Zitz, Jacob E (22)
|
NIVICT |
24.18 |
25.06 |
0.88 |
| 3 |
Chasman, Aidan P (13)
|
NIVICT |
25.95 |
25.88 |
-0.07 |
| 4 |
Caswell, Christopher R (13)
|
NIGCOM |
28.47 |
27.01 |
-1.46 |
| 5 |
Alexander, Nathan R (13)
|
NIJETS |
27.30 |
27.12 |
-0.18 |
| 6 |
Culhane, Liam M (14)
|
NIVICT |
29.74 |
27.38 |
-2.36 |
| 7 |
Vitale, Christian R (13)
|
NIVICT |
29.05 |
28.09 |
-0.96 |
| 8 |
Stevens, Jonathan P (13)
|
NIJETS |
31.39 |
29.90 |
-1.49 |
| 9 |
DiGiacomo, Dominic J (12)
|
NICLSC |
31.23 |
30.43 |
-0.80 |
| 10 |
Gockeler Schultz, Jonathan S (13)
|
NIVICT |
33.02 |
30.57 |
-2.45 |
| 11 |
Green, William E (14)
|
NIHAST |
30.97 |
31.38 |
0.41 |
| 12 |
Newsome, George W (11)
|
NIPMSC |
32.55 |
32.84 |
0.29 |