| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hawkins, Emma (14)
|
NIVICT |
55.91 |
58.10 |
2.19 |
| 2 |
Park, Rebecca (14)
|
NIVICT |
57.18 |
58.36 |
1.18 |
| 3 |
Senglaub, Emma (14)
|
NIVICT |
56.60 |
58.92 |
2.32 |
| 4 |
Pathammavong, Suriyathai A (13)
|
NIVICT |
58.33 |
58.95 |
0.62 |
| 5 |
Werges, Lillian F (13)
|
NIVICT |
1:01.81 |
1:01.09 |
-0.72 |
| 6 |
Patterson, Ellen R (14)
|
NIIRON |
1:04.50 |
1:02.07 |
-2.43 |
| 7 |
Grego, Liliya E (14)
|
NIGCOM |
1:06.44 |
1:05.56 |
-0.88 |
| 8 |
Wallace, Emma E (14)
|
NIGRAT |
1:02.92 |
1:05.98 |
3.06 |
| 9 |
Caldwell, Hannah M (12)
|
NIVICT |
1:05.32 |
1:06.26 |
0.94 |
| 10 |
Martin, Lauren G (12)
|
NISDSC |
1:10.05 |
1:08.09 |
-1.96 |
| 11 |
Cassidy, Alexa E (13)
|
NISDSC |
1:18.23 |
1:09.93 |
-8.30 |
| 12 |
Cywinski, Anna C (10)
|
NIVICT |
1:11.04 |
1:10.01 |
-1.03 |
| 13 |
Werner, Riley K (13)
|
NIVICT |
1:11.35 |
1:10.76 |
-0.59 |
| 14 |
Prezyna, Zoey (10)
|
NIVICT |
1:08.67 |
1:11.32 |
2.65 |
| 15 |
Onze, Lauren W (11)
|
NIVICT |
1:13.62 |
1:12.40 |
-1.22 |
| 16 |
Stepanek, Abbigail L (13)
|
NIHAST |
1:14.79 |
1:12.65 |
-2.14 |
| 17 |
Christodaro, Raena M (13)
|
NIHAST |
1:15.93 |
1:12.95 |
-2.98 |
| 18 |
Bennett, Olivia R (11)
|
NICAQY |
1:22.06 |
1:20.94 |
-1.12 |
|
Bolte-Pauley, Ashlyn R (14)
|
NIGRAT |
|
NS |
- |