| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ludlow, Benjamin L (17)
|
NIMERC |
53.36 |
55.94 |
2.58 |
| 2 |
Morgan, Phillip A (16)
|
NIMERC |
58.37 |
58.76 |
0.39 |
| 3 |
Anderson, Carter R (16)
|
NIISWM |
57.07 |
59.58 |
2.51 |
| 4 |
Kreiley, Aidan C (16)
|
NIMERC |
1:01.64 |
59.69 |
-1.95 |
| 5 |
Morreale, Alexander H (15)
|
NIHAST |
1:00.65 |
1:00.56 |
-0.09 |
| 6 |
Matthews III, Charles S (17)
|
NIMERC |
1:00.40 |
1:00.65 |
0.25 |
| 7 |
Glaubitz, Flaminio S (16)
|
NIISWM |
57.13 |
1:01.18 |
4.05 |
| 8 |
D'Alessandro, Kai J (16)
|
NIVICT |
1:01.57 |
1:03.05 |
1.48 |
| 9 |
Zitz, Jacob E (22)
|
NIVICT |
57.12 |
1:05.51 |
8.39 |
| 10 |
Currie, Justin A (16)
|
NIWWSC |
1:10.55 |
1:05.65 |
-4.90 |
| 11 |
Michalowski, Connor J (16)
|
NIHAST |
1:06.80 |
1:07.55 |
0.75 |
| 12 |
Ginder, Fredrick (17)
|
NIVICT |
1:09.33 |
1:08.72 |
-0.61 |
| 13 |
Smith, Connor J (15)
|
NIVICT |
1:07.36 |
1:10.51 |
3.15 |
| 14 |
Labue, Payton M (16)
|
NIHAST |
1:10.54 |
1:11.34 |
0.80 |