| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Deuel, Megan G (15)
|
NIVICT |
24.45 |
25.92 |
1.47 |
| 2 |
Guagliardo, Gabriella N (17)
|
NIIRON |
26.22 |
26.64 |
0.42 |
| 3 |
Regelsberger, Emily C (15)
|
NIVICT |
25.82 |
26.87 |
1.05 |
| 4 |
Ware, Kendall E (17)
|
NIVICT |
26.69 |
27.29 |
0.60 |
| 5 |
Branch, Alison G (16)
|
NIIRON |
27.60 |
27.34 |
-0.26 |
| 6 |
Thurston, Sophia R (17)
|
NIIRON |
28.00 |
27.54 |
-0.46 |
| 7 |
Berkhof, Lucia M (17)
|
NIVICT |
25.33 |
27.59 |
2.26 |
| 8 |
Farman, Morgan K (18)
|
NIVICT |
26.45 |
27.69 |
1.24 |
| 9 |
DiFante, Marlayna R (16)
|
NIGCOM |
27.48 |
28.57 |
1.09 |
| 10 |
Christodaro, Melina A (16)
|
NIHAST |
26.93 |
28.86 |
1.93 |
| 11 |
Stepanek, Livia G (15)
|
NIHAST |
28.48 |
29.11 |
0.63 |
| 12 |
Kennedy, Madaliene F (15)
|
NISDSC |
30.69 |
29.59 |
-1.10 |
| 13 |
Neubecker, Sarah C (17)
|
NICAQY |
28.50 |
29.88 |
1.38 |
| 14 |
Chadwick, Alina V (15)
|
NISDSC |
28.96 |
30.86 |
1.90 |
| 15 |
Geoca, Zoey L (15)
|
NISDSC |
30.21 |
31.00 |
0.79 |
|
Pollock, Kathleen C (17)
|
NIVICT |
23.84 |
NS |
- |