| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wisner, Taryn A (16)
|
NIVICT |
52.78 |
55.47 |
2.69 |
| 2 |
Crye, Joleigh C (14)
|
NIMERC |
54.10 |
55.50 |
1.40 |
| 3 |
Hawkins, Emma (15)
|
NIVICT |
54.49 |
56.47 |
1.98 |
| 4 |
Park, Rebecca (15)
|
NIVICT |
54.85 |
56.99 |
2.14 |
| 5 |
Kidder, Elizabeth A (15)
|
NIHAST |
55.14 |
57.81 |
2.67 |
| 6 |
Gellert, Delaney E (15)
|
NIJETS |
56.12 |
58.38 |
2.26 |
| 7 |
Senglaub, Emma (15)
|
NIVICT |
54.93 |
58.47 |
3.54 |
| 8 |
Pathammavong, Suriyathai A (14)
|
NIVICT |
56.72 |
58.82 |
2.10 |
| 9 |
Konz, Ashley L (14)
|
NIJETS |
58.09 |
58.90 |
0.81 |
| 10 |
Senglaub, Katie (15)
|
NIVICT |
58.06 |
59.55 |
1.49 |
| 11 |
Werges, Lillian F (14)
|
NIVICT |
59.92 |
1:00.19 |
0.27 |
| 12 |
LeFrois-Heath, Emily R (14)
|
NIVICT |
58.42 |
1:01.16 |
2.74 |
| 13 |
McRae, Kathryn E (13)
|
NIVICT |
1:04.52 |
1:02.47 |
-2.05 |
| 14 |
Russo, Sophia E (15)
|
NIJETS |
|
1:03.46 |
- |
| 15 |
Geoca, Zoey L (16)
|
NISDSC |
1:02.53 |
1:04.09 |
1.56 |
| 16 |
Trapp, Angelina L (13)
|
NIPMSC |
1:03.89 |
1:04.38 |
0.49 |
| 17 |
Kazmark, Raelyn J (14)
|
NIVICT |
1:04.18 |
1:04.48 |
0.30 |
| 18 |
Gellert, Emerson P (13)
|
NIJETS |
1:04.03 |
1:05.11 |
1.08 |
| 19 |
de Ruijter, Domenique (14)
|
NIJETS |
1:03.12 |
1:05.33 |
2.21 |
| 20 |
Berdysiak, Elizabeth C (13)
|
NICLSC |
1:05.53 |
1:05.60 |
0.07 |
| 21 |
Goetzke, Emma G (16)
|
NIJETS |
1:05.97 |
1:05.63 |
-0.34 |
| 22 |
Wilck, Emilena R (14)
|
NINEWW |
1:06.47 |
1:06.63 |
0.16 |
| 23 |
Pierce, Marek J (12)
|
NINEWW |
1:11.17 |
1:06.75 |
-4.42 |
| 24 |
Rouse, Ellie R (13)
|
NISDSC |
1:20.09 |
1:13.81 |
-6.28 |
| 25 |
Selioutski, Leeva A (14)
|
NISDSC |
1:31.29 |
1:13.90 |
-17.39 |
| 26 |
Kenny, Elisabeth A (13)
|
NIVICT |
1:22.18 |
1:16.66 |
-5.52 |
|
Werner, Riley K (14)
|
NIVICT |
1:10.56 |
NS |
- |