| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Melchior, Ava G (6)
|
MAGPAC |
|
23.20 |
- |
| 2 |
Fleck, Veronica J (6)
|
MATHY |
26.95 |
25.67 |
-1.28 |
| 3 |
Ruslim, Eva (6)
|
MAWAC |
27.32 |
26.64 |
-0.68 |
| 4 |
Jones, Madeleine H (6)
|
MAWAC |
28.14 |
27.71 |
-0.43 |
| 5 |
Kalinowski, Lillian R (6)
|
MAWAC |
26.10 |
28.46 |
2.36 |
| 6 |
Scott, Julia T (5)
|
MASJAC |
31.50 |
28.57 |
-2.93 |
| 7 |
Wark, Tessa R (6)
|
MAWAC |
34.22 |
30.04 |
-4.18 |
| 8 |
Hutton, Ellie R (6)
|
MAWAC |
|
31.35 |
- |
| 9 |
Boyer, Emma L (6)
|
MAWAC |
37.00 |
33.32 |
-3.68 |
| 10 |
Westcott, Charlotte M (6)
|
MAWAC |
|
33.69 |
- |
| 11 |
Headrick, Lucy F (6)
|
MASJAC |
38.00 |
34.56 |
-3.44 |
| 12 |
Smith, Tristyn S (5)
|
MAGPAC |
36.19 |
35.20 |
-0.99 |
| 13 |
Hauske, Megan L (5)
|
MAWAC |
35.36 |
35.92 |
0.56 |