| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holcroft, Lucy L (6)
|
MASJAC |
|
24.57 |
- |
| 2 |
Scott, Julia T (6)
|
MASJAC |
27.51 |
26.49 |
-1.02 |
| 3 |
Jauffret, Clementine F (6)
|
MAWAC |
|
26.65 |
- |
| 4 |
Hauske, Megan L (6)
|
MAWAC |
30.99 |
26.75 |
-4.24 |
| 5 |
Pasquini, Aubrey E (6)
|
MASJAC |
26.71 |
27.24 |
0.53 |
| 6 |
Gaynor, Juliet E (6)
|
MASJAC |
29.46 |
28.02 |
-1.44 |
| 7 |
Smith, Tristyn S (6)
|
MAGPAC |
30.82 |
28.10 |
-2.72 |
| 8 |
Gurcik, Alyssa F (6)
|
MASJAC |
27.41 |
28.16 |
0.75 |
| 9 |
Geist, Helena S (5)
|
MASJAC |
|
29.13 |
- |
| 10 |
Cole, Emma F (6)
|
MAWAC |
|
29.24 |
- |
| 11 |
Grant, Jayden C (6)
|
MAWAC |
29.65 |
29.86 |
0.21 |
| 12 |
Melchior, Ella R (5)
|
MAGPAC |
30.72 |
32.34 |
1.62 |
| 13 |
Weag, Emilia C (6)
|
MASJAC |
32.29 |
32.51 |
0.22 |
| 14 |
Priestley, Bridget (5)
|
MAWAC |
|
33.28 |
- |
| 15 |
Jackson, Chloe E (5)
|
MAWAC |
|
33.96 |
- |
| 16 |
DuBois, Morgan E (6)
|
MAGPAC |
|
35.03 |
- |
| 17 |
Gateretse, Isabelle L (4)
|
MAWAC |
39.35 |
37.76 |
-1.59 |
| 18 |
Giordano, Charlotte M (5)
|
MAWAC |
|
40.38 |
- |
| 19 |
Alberto, Emerson J (5)
|
MAWAC |
1:08.82 |
40.70 |
-28.12 |
| 20 |
Fillioe, Allison G (5)
|
MAWAC |
|
50.42 |
- |
|
O'Brien, Emma R (6)
|
MAWAC |
|
NS |
- |