| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stewart, Julian A (8)
|
MASJAC |
44.57 |
42.85 |
-1.72 |
| 2 |
Long, Francis A (8)
|
MAFSA |
43.96 |
43.24 |
-0.72 |
| 3 |
Stankus, Ryan F (8)
|
MASJAC |
50.96 |
44.65 |
-6.31 |
| 4 |
Stride, Nicholas D (8)
|
MASJAC |
|
44.69 |
- |
| 5 |
Clark, Lucas S (7)
|
MASJAC |
53.50 |
45.79 |
-7.71 |
| 6 |
Gill, Raunak S (8)
|
MAT85 |
46.82 |
46.22 |
-0.60 |
| 7 |
Metzler, Dylan A (8)
|
MASJAC |
48.26 |
46.62 |
-1.64 |
| 8 |
Valentino, Frederick J (8)
|
MASJAC |
|
49.90 |
- |
| 9 |
Mancinelli, Brayden L (8)
|
MASJAC |
57.84 |
50.69 |
-7.15 |
| 10 |
Massott, Jackson R (7)
|
MASJAC |
1:06.21 |
51.23 |
-14.98 |
| 11 |
Boone, Matthew S (8)
|
MASJAC |
1:01.82 |
55.55 |
-6.27 |
| 12 |
Henderson, Gabriel R (7)
|
MAUNSJ |
59.20 |
57.48 |
-1.72 |
| 13 |
Bruder, Derek A (8)
|
MASJAC |
|
58.02 |
- |
| 14 |
Lazaro, Lucas (7)
|
MASJAC |
|
59.05 |
- |
| 15 |
Balog, Colton (8)
|
MAT85 |
1:04.95 |
1:02.85 |
-2.10 |
| 16 |
Belkin, Blake L (8)
|
MASJAC |
|
1:09.88 |
- |